BMRB Entry 27165
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27165
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kieken, Fabien; Loth, Karine; Van Nuland, Nico; Tompa, Peter; Lenaerts, TOM. "Chemical shift assignments of the partially deuterated Fyn SH2-SH3 domain" Biomol. NMR Assign. 12, 117-122 (2018).
PubMed: 29224116
Assembly members:
Fyn_Human_SH3-SH2, polymer, 171 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15b
Entity Sequences (FASTA):
Fyn_Human_SH3-SH2: GSHMTGVTLFVALYDYEART
EDDLSFHKGEKFQILNSSEG
DWWEARSLTTGETGYIPSNY
VAPVDSIQAEEWYFGKLGRK
DAERQLLSFGNPRGTFLIRE
SETTKGAYSLSIRDWDDMKG
DHVKHYKIRKLDNGGYYITT
RAQFETLQQLVQHYSERAAG
LCCRLVVPCHK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 750 |
| 15N chemical shifts | 179 |
| 1H chemical shifts | 1160 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fyn-SH3SH2 | 1 |
Entities:
Entity 1, Fyn-SH3SH2 171 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | THR | GLY | VAL | THR | LEU | PHE | ||||
| 2 | VAL | ALA | LEU | TYR | ASP | TYR | GLU | ALA | ARG | THR | ||||
| 3 | GLU | ASP | ASP | LEU | SER | PHE | HIS | LYS | GLY | GLU | ||||
| 4 | LYS | PHE | GLN | ILE | LEU | ASN | SER | SER | GLU | GLY | ||||
| 5 | ASP | TRP | TRP | GLU | ALA | ARG | SER | LEU | THR | THR | ||||
| 6 | GLY | GLU | THR | GLY | TYR | ILE | PRO | SER | ASN | TYR | ||||
| 7 | VAL | ALA | PRO | VAL | ASP | SER | ILE | GLN | ALA | GLU | ||||
| 8 | GLU | TRP | TYR | PHE | GLY | LYS | LEU | GLY | ARG | LYS | ||||
| 9 | ASP | ALA | GLU | ARG | GLN | LEU | LEU | SER | PHE | GLY | ||||
| 10 | ASN | PRO | ARG | GLY | THR | PHE | LEU | ILE | ARG | GLU | ||||
| 11 | SER | GLU | THR | THR | LYS | GLY | ALA | TYR | SER | LEU | ||||
| 12 | SER | ILE | ARG | ASP | TRP | ASP | ASP | MET | LYS | GLY | ||||
| 13 | ASP | HIS | VAL | LYS | HIS | TYR | LYS | ILE | ARG | LYS | ||||
| 14 | LEU | ASP | ASN | GLY | GLY | TYR | TYR | ILE | THR | THR | ||||
| 15 | ARG | ALA | GLN | PHE | GLU | THR | LEU | GLN | GLN | LEU | ||||
| 16 | VAL | GLN | HIS | TYR | SER | GLU | ARG | ALA | ALA | GLY | ||||
| 17 | LEU | CYS | CYS | ARG | LEU | VAL | VAL | PRO | CYS | HIS | ||||
| 18 | LYS |
Samples:
sample_1: Fyn Human SH3-SH2, U-100% 15N, U-100% 13C, 60% 2H, 800 uM; sodium phosphate 50 mM; sodium sulfate 100 mM; B mercapto ethanol 2 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN, Bruker Biospin - processing
CCPNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks