BMRB Entry 27162

Name: NMR resonance assignments of the EVH1-domain of Neurofibromin&#039;s recruitment factor Spred1
Published: 2017-09-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27162

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR resonance assignments of the EVH1-domain of Neurofibromin's recruitment factor Spred1

Deposition date:

2017-06-26

Original release date:

2017-09-29

Authors:

Fuehrer, Sebastian; Ahammer, Linda; Ausserbichler, Angela; Scheffzek, Klaus; Dunzendorfer-Matt, Theresia; Tollinger, Martin

Citation:

Citation: Fuehrer, Sebastian; Ahammer, Linda; Ausserbichler, Angela; Scheffzek, Klaus; Dunzendorfer-Matt, Theresia; Tollinger, Martin. "NMR resonance assignments of the EVH1 domain of neurofibromin's recruitment factor Spred1" Biomol. NMR Assignments 11, 305-308 (2017).
PubMed: 28831766

Assembly members:

Assembly members:
Spred1-EVH1, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Spred1-EVH1: MGSYARVRAVVMTRDDSSGG WLPLGGSGLSSVTVFKVPHQ EENGCADFFIRGERLRDKMV VLECMLKKDLIYNKVTPTFH HWKIDDKKFGLTFQSPADAR AFDRGIRRAIEDISQGCPES

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	461
15N chemical shifts	117
1H chemical shifts	723

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Spred1-EVH1	1

Entities:

Entity 1, Spred1-EVH1 120 residues - Formula weight is not available

The first two residues, Met-Gly, are derived from the plasmid

1	MET	GLY	SER	TYR	ALA	ARG	VAL	ARG	ALA	VAL
2	VAL	MET	THR	ARG	ASP	ASP	SER	SER	GLY	GLY
3	TRP	LEU	PRO	LEU	GLY	GLY	SER	GLY	LEU	SER
4	SER	VAL	THR	VAL	PHE	LYS	VAL	PRO	HIS	GLN
5	GLU	GLU	ASN	GLY	CYS	ALA	ASP	PHE	PHE	ILE
6	ARG	GLY	GLU	ARG	LEU	ARG	ASP	LYS	MET	VAL
7	VAL	LEU	GLU	CYS	MET	LEU	LYS	LYS	ASP	LEU
8	ILE	TYR	ASN	LYS	VAL	THR	PRO	THR	PHE	HIS
9	HIS	TRP	LYS	ILE	ASP	ASP	LYS	LYS	PHE	GLY
10	LEU	THR	PHE	GLN	SER	PRO	ALA	ASP	ALA	ARG
11	ALA	PHE	ASP	ARG	GLY	ILE	ARG	ARG	ALA	ILE
12	GLU	ASP	ILE	SER	GLN	GLY	CYS	PRO	GLU	SER

Samples:

sample_1: Spred1-EVH1, [U-99% 13C; U-99% 15N], 0.6-0.8 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CC(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CC)(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Agilent DD2 500 MHz

1	MET	GLY	SER	TYR	ALA	ARG	VAL	ARG	ALA	VAL
2	VAL	MET	THR	ARG	ASP	ASP	SER	SER	GLY	GLY
3	TRP	LEU	PRO	LEU	GLY	GLY	SER	GLY	LEU	SER
4	SER	VAL	THR	VAL	PHE	LYS	VAL	PRO	HIS	GLN
5	GLU	GLU	ASN	GLY	CYS	ALA	ASP	PHE	PHE	ILE
6	ARG	GLY	GLU	ARG	LEU	ARG	ASP	LYS	MET	VAL
7	VAL	LEU	GLU	CYS	MET	LEU	LYS	LYS	ASP	LEU
8	ILE	TYR	ASN	LYS	VAL	THR	PRO	THR	PHE	HIS
9	HIS	TRP	LYS	ILE	ASP	ASP	LYS	LYS	PHE	GLY
10	LEU	THR	PHE	GLN	SER	PRO	ALA	ASP	ALA	ARG
11	ALA	PHE	ASP	ARG	GLY	ILE	ARG	ARG	ALA	ILE
12	GLU	ASP	ILE	SER	GLN	GLY	CYS	PRO	GLU	SER

1	MET	GLY	SER	TYR	ALA	ARG	VAL	ARG	ALA	VAL
2	VAL	MET	THR	ARG	ASP	ASP	SER	SER	GLY	GLY
3	TRP	LEU	PRO	LEU	GLY	GLY	SER	GLY	LEU	SER
4	SER	VAL	THR	VAL	PHE	LYS	VAL	PRO	HIS	GLN
5	GLU	GLU	ASN	GLY	CYS	ALA	ASP	PHE	PHE	ILE
6	ARG	GLY	GLU	ARG	LEU	ARG	ASP	LYS	MET	VAL
7	VAL	LEU	GLU	CYS	MET	LEU	LYS	LYS	ASP	LEU
8	ILE	TYR	ASN	LYS	VAL	THR	PRO	THR	PHE	HIS
9	HIS	TRP	LYS	ILE	ASP	ASP	LYS	LYS	PHE	GLY
10	LEU	THR	PHE	GLN	SER	PRO	ALA	ASP	ALA	ARG
11	ALA	PHE	ASP	ARG	GLY	ILE	ARG	ARG	ALA	ILE
12	GLU	ASP	ILE	SER	GLN	GLY	CYS	PRO	GLU	SER