BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27159

Title: LRT2 Cyclophilin of rice   PubMed: 29353448

Deposition date: 2017-06-22 Original release date: 2018-02-02

Authors: Acevedo, Lucila; Nicholson, Linda

Citation: Acevedo, Lucila; Nicholson, Linda. "1H, 13C and 15N NMR assignments of cyclophilin LRT2 (OsCYP2) from rice"  Biomol. NMR Assignments 12, 171-174 (2018).

Assembly members:
LRT2, polymer, 175 residues, 18686.36 Da.

Natural source:   Common Name: rice   Taxonomy ID: 4530   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oryza sativa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet28

Entity Sequences (FASTA):
LRT2: GHMMSNTRVFFDMTVGGAPA GRIVMELYAKDVPRTAENFR ALCTGEKGVGKSGKPLHYKG STFHRVIPEFMCQGGDFTRG NGTGGESIYGEKFADEVFKF KHDSPGILSMANAGPNTNGS QFFICTVPCSWLDGKHVVFG RVVEGMDVVKAIEKVGSRGG STAKPVVIADCGQLS

Data sets:
Data typeCount
13C chemical shifts638
15N chemical shifts164
1H chemical shifts983

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LRT21

Entities:

Entity 1, LRT2 175 residues - 18686.36 Da.

residue 1 is left after TEV cleavage and residues 2 and 3 represent cloning artifacts

1   GLYHISMETMETSERASNTHRARGVALPHE
2   PHEASPMETTHRVALGLYGLYALAPROALA
3   GLYARGILEVALMETGLULEUTYRALALYS
4   ASPVALPROARGTHRALAGLUASNPHEARG
5   ALALEUCYSTHRGLYGLULYSGLYVALGLY
6   LYSSERGLYLYSPROLEUHISTYRLYSGLY
7   SERTHRPHEHISARGVALILEPROGLUPHE
8   METCYSGLNGLYGLYASPPHETHRARGGLY
9   ASNGLYTHRGLYGLYGLUSERILETYRGLY
10   GLULYSPHEALAASPGLUVALPHELYSPHE
11   LYSHISASPSERPROGLYILELEUSERMET
12   ALAASNALAGLYPROASNTHRASNGLYSER
13   GLNPHEPHEILECYSTHRVALPROCYSSER
14   TRPLEUASPGLYLYSHISVALVALPHEGLY
15   ARGVALVALGLUGLYMETASPVALVALLYS
16   ALAILEGLULYSVALGLYSERARGGLYGLY
17   SERTHRALALYSPROVALVALILEALAASP
18   CYSGLYGLNLEUSER

Samples:

sample_1: LRT2, [U-99% 13C; U-99% 15N], 1.16 mM; potassium chloride 50 mM; potassium phosphate 20 mM; TCEP 1 mM; sodium azide 5 mM

sample_conditions_1: ionic strength: 94.76 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

NCBI BAS76709

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts