BMRB Entry 27159

Name: LRT2 Cyclophilin of rice
Published: 2018-02-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27159

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

LRT2 Cyclophilin of rice

Deposition date:

2017-06-22

Original release date:

2018-02-02

Authors:

Acevedo, Lucila; Nicholson, Linda

Citation:

Citation: Acevedo, Lucila; Nicholson, Linda. "1H, 13C and 15N NMR assignments of cyclophilin LRT2 (OsCYP2) from rice" Biomol. NMR Assignments 12, 171-174 (2018).
PubMed: 29353448

Assembly members:

Assembly members:
LRT2, polymer, 175 residues, 18686.36 Da.

Natural source:

Natural source: Common Name: rice Taxonomy ID: 4530 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oryza sativa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LRT2: GHMMSNTRVFFDMTVGGAPA GRIVMELYAKDVPRTAENFR ALCTGEKGVGKSGKPLHYKG STFHRVIPEFMCQGGDFTRG NGTGGESIYGEKFADEVFKF KHDSPGILSMANAGPNTNGS QFFICTVPCSWLDGKHVVFG RVVEGMDVVKAIEKVGSRGG STAKPVVIADCGQLS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	638
15N chemical shifts	164
1H chemical shifts	983

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LRT2	1

Entities:

Entity 1, LRT2 175 residues - 18686.36 Da.

residue 1 is left after TEV cleavage and residues 2 and 3 represent cloning artifacts

1

GLY

HIS

MET

SER

ASN

THR

ARG

VAL

PHE

2

PHE

ASP

MET

THR

VAL

GLY

ALA

PRO

ALA

3

GLY

ARG

ILE

VAL

MET

GLU

LEU

TYR

ALA

LYS

4

ASP

VAL

PRO

ARG

THR

ALA

GLU

ASN

PHE

ARG

5

ALA

LEU

CYS

THR

GLY

GLU

LYS

GLY

VAL

GLY

6

LYS

SER

GLY

LYS

PRO

LEU

HIS

TYR

LYS

GLY

7

SER

THR

PHE

HIS

ARG

VAL

ILE

PRO

GLU

PHE

8

MET

CYS

GLN

GLY

ASP

PHE

THR

ARG

GLY

9

ASN

GLY

THR

GLY

GLU

SER

ILE

TYR

GLY

10

GLU

LYS

PHE

ALA

ASP

GLU

VAL

PHE

LYS

PHE

11

LYS

HIS

ASP

SER

PRO

GLY

ILE

LEU

SER

MET

12

ALA

ASN

ALA

GLY

PRO

ASN

THR

ASN

GLY

SER

13

GLN

PHE

ILE

CYS

THR

VAL

PRO

CYS

SER

14

TRP

LEU

ASP

GLY

LYS

HIS

VAL

PHE

GLY

15

ARG

VAL

GLU

GLY

MET

ASP

VAL

LYS

16

ALA

ILE

GLU

LYS

VAL

GLY

SER

ARG

GLY

17

SER

THR

ALA

LYS

PRO

VAL

ILE

ALA

ASP

18

CYS

GLY

GLN

LEU

SER

Samples:

sample_1: LRT2, [U-99% 13C; U-99% 15N], 1.16 mM; potassium chloride 50 mM; potassium phosphate 20 mM; TCEP 1 mM; sodium azide 5 mM

sample_conditions_1: ionic strength: 94.76 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Varian INOVA 600 MHz