BMRB Entry 27152

Name: 3Jcgn &amp; 3Jcgc&#039; scalar couplings for CUG-BP2 RRM3 H429A mutant in complex with (5&#039;-R(*UP*UP*UP*AP*A)-3&#039;)
Published: 2017-09-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27152

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

3Jcgn & 3Jcgc' scalar couplings for CUG-BP2 RRM3 H429A mutant in complex with (5'-R(*UP*UP*UP*AP*A)-3')

Deposition date:

2017-06-21

Original release date:

2017-09-29

Authors:

Diarra dit Konte, N.; Damberger, F.; Allain, F.

Citation:

Citation: Diarra Dit Konte, Nana; Krepl, Miroslav; Damberger, Fred; Ripin, Nina; Duss, Olivier; Sponer, Jiri; Allain, Frederic H-T. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination" Nat. Commun. 8, 654-654 (2017).
PubMed: 28935965

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available
entity_2, polymer, 5 residues, 1531.952 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MQKEGPEGANLFIYALPQEF GDQDILQMFMPFGNVISAKV FIDKQTNLSKCFGFVSYDNP VSAQAAIQAMNGFQIGMKRL KVQLKRSKNDSKPW
entity_2: UUUAA

Data sets:

coupling_constants
- coupling_constants_1

Data type	Count
coupling constants	19

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 114 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLN

LYS

GLU

GLY

PRO

GLU

GLY

ALA

ASN

4

LEU

PHE

ILE

TYR

ALA

LEU

PRO

GLN

GLU

PHE

5

GLY

ASP

GLN

ASP

ILE

LEU

GLN

MET

PHE

MET

6

PRO

PHE

GLY

ASN

VAL

ILE

SER

ALA

LYS

VAL

7

PHE

ILE

ASP

LYS

GLN

THR

ASN

LEU

SER

LYS

8

CYS

PHE

GLY

PHE

VAL

SER

TYR

ASP

ASN

PRO

9

VAL

SER

ALA

GLN

ALA

ILE

GLN

ALA

MET

10

ASN

GLY

PHE

GLN

ILE

GLY

MET

LYS

ARG

LEU

11

LYS

VAL

GLN

LEU

LYS

ARG

SER

LYS

ASN

ASP

12

SER

LYS

PRO

TRP

Entity 2, entity_2 5 residues - 1531.952 Da.

1

U

A

Samples:

sample_1: 5'-UUUAA-3' RNA, non-labeled, 1.95 mM; CUG-BP2 third RNA Recognition Motif, [U-99% 13C; U-99% 15N], 0.65 mM; H2O 95 % v/v; D2O 5 % v/v; potassium phosphate 20 mM; sodium chloride 10 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v

sample_conditions_1: ionic strength: 130 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
spin-echo difference constant time 15N-HSQC	sample_1	isotropic	sample_conditions_1
spin-echo difference constant time HN(CO)CG	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker AvanceIII 600 MHz
Bruker AvanceIII HD 600 MHz