BMRB Entry 27140

Name: 3Jcgn &amp; 3Jcgc&#039; scalar couplings for CUG-BP2 RRM3 wild type
Published: 2017-09-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27140

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

3Jcgn & 3Jcgc' scalar couplings for CUG-BP2 RRM3 wild type

Deposition date:

2017-06-15

Original release date:

2017-09-29

Authors:

Damberger, Fred

Citation:

Citation: Diarra Dit Konte, Nana; Krepl, Miroslav; Damberger, Fred; Ripin, Nina; Duss, Olivier; Sponer, Jiri; Allain, Frederic H-T. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination" Nat. Commun. 8, 654-654 (2017).
PubMed: 28935965

Assembly members:

Assembly members:
CUG_BP2_RRM3, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CUG_BP2_RRM3: MGSSHHHHHHSSGLVPRGSH MQKEGPEGANLFIYHLPQEF GDQDILQMFMPFGNVISAKV FIDKQTNLSKCFGFVSYDNP VSAQAAIQAMNGFQIGMKRL KVQLKRSKNDSKPW

Data sets:

coupling_constants
- coupling_constant_list_1

Data type	Count
coupling constants	20

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM3	1

Entities:

Entity 1, RRM3 114 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLN

LYS

GLU

GLY

PRO

GLU

GLY

ALA

ASN

4

LEU

PHE

ILE

TYR

HIS

LEU

PRO

GLN

GLU

PHE

5

GLY

ASP

GLN

ASP

ILE

LEU

GLN

MET

PHE

MET

6

PRO

PHE

GLY

ASN

VAL

ILE

SER

ALA

LYS

VAL

7

PHE

ILE

ASP

LYS

GLN

THR

ASN

LEU

SER

LYS

8

CYS

PHE

GLY

PHE

VAL

SER

TYR

ASP

ASN

PRO

9

VAL

SER

ALA

GLN

ALA

ILE

GLN

ALA

MET

10

ASN

GLY

PHE

GLN

ILE

GLY

MET

LYS

ARG

LEU

11

LYS

VAL

GLN

LEU

LYS

ARG

SER

LYS

ASN

ASP

12

SER

LYS

PRO

TRP

Samples:

sample_1: CUG BP2 RRM3, [U-100% 13C; U-100% 15N], 0.65 mM; D2O, [U-100% 2H], 5 % v/v; H2O 95 % v/v; sodium chloride 10 mM; potassium phosphate 20 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
spin-echo difference constant time 15N-HSQC	sample_1	isotropic	sample_conditions_1
spin-echo difference constant time HN(CO)CG	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 600 MHz