BMRB Entry 27134

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27134

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
C-terminal domain of merozoite surface protein 2
Deposition date:
2017-06-11
Original release date:
2017-06-15
Authors:
Das, Sreedam; Morales, Rodrigo; Seow, Jeffrey; Bankala, Krishnarjuna; Dissanayake, Ravindu; Anders, Robin; MacRaild, Christopher; Norton, Raymond
Citation:

Citation: Das, Sreedam; Morales, Rodrigo; Seow, Jeffrey; Krishnarjuna, Bankala; Dissanayake, Ravindu; Anders, Robin; MacRaild, Christopher; Norton, Raymond. "Lipid interactions modulate the structural and antigenic properties of the C-terminal domain of the malaria antigen merozoite surface protein 2"  FEBS J. 284, 2649-2662 (2017).
PubMed: 28618199

Assembly members:

Assembly members:
C-terminal_domain_of_MSP2, polymer, 58 residues, 6300 Da.

Natural source:

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal_domain_of_MSP2: APENKGTGQHGHMHGSRNNH PQNTSDSQKECTDGNKENCG AATSLLSNSSLEHHHHHH

Data typeCount
13C chemical shifts196
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MSP2 (172-221)1

Entities:

Entity 1, MSP2 (172-221) 58 residues - 6300 Da.

First 50 resides are conserved C-terminal MSP2 and this construct has two extra residues (Leu, Glu) from the vector between the protein and His6-tag, giving a total of 58 residues.

1   ALAPROGLUASNLYSGLYTHRGLYGLNHIS
2   GLYHISMETHISGLYSERARGASNASNHIS
3   PROGLNASNTHRSERASPSERGLNLYSGLU
4   CYSTHRASPGLYASNLYSGLUASNCYSGLY
5   ALAALATHRSERLEULEUSERASNSERSER
6   LEUGLUHISHISHISHISHISHIS

Samples:

sample_4: C-terminal region of MSP2, [U-99% 13C; U-98% 15N], 1.25 mM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v; Dodecylphosphocholine, [U-98% 2H], 200 mM; DOGS-NTA 2 mM; Nickel sulphate 1.5 mM

sample_1: C-terminal domain of MSP2, [U-98% 15N], 100 uM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v

sample_2: C-terminal domain of MSP2, [U-98% 15N], 100 uM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v; DPC, [U-98% 2H], 100 mM; DOGS-NTA 1 mM; Nickel sulphate 0.2 mM

sample_3: C-terminal domain of MSP2, [U-99% 13C; U-98% 15N], 1.25 mM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v

sample_conditions_1: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D HNCOCAsample_4isotropicsample_conditions_1

Software:

TOPSPIN v2.3, Bruker Biospin - collection, processing

CcpNMR v2.4.2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance lll 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks