BMRB Entry 27127

Name: 1H, 13C, 15N Chemical Shift Assignments for vaccinia topoisomerase 1B N terminal domain (1-80)
Published: 2017-06-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27127

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Chemical Shift Assignments for vaccinia topoisomerase 1B N terminal domain (1-80)

Deposition date:

2017-06-06

Original release date:

2017-06-19

Authors:

Reed, Benjamin

Citation:

Citation: Reed, Benjamin; Yakovleva, Lyudmila; Shuman, Stewart; Ghose, Ranajeet. "Characterization of DNA Binding by the Isolated N-Terminal Domain of Vaccinia Virus DNA Topoisomerase IB" Biochemistry 56, 3307-3317 (2017).
PubMed: 28570045

Assembly members:

Assembly members:
TopN, polymer, 82 residues, 9596.8 Da.

Natural source:

Natural source: Common Name: Vaccinia virus Taxonomy ID: 10254 Superkingdom: Poxviridae Kingdom: Chordopoxvirinae Genus/species: Orthopoxvirus vaccinia

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TopN: GSMRALFYKDGKLFTDNNFL NPVSDDNPAYEVLQHVKIPT HLTDVVVYEQTWEEALTRLI FVGSDSKGRRQYFYGKMHVQ NR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	315
15N chemical shifts	77
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TopN monomer	1

Entities:

Entity 1, TopN monomer 82 residues - 9596.8 Da.

1

GLY

SER

MET

ARG

ALA

LEU

PHE

TYR

LYS

ASP

2

GLY

LYS

LEU

PHE

THR

ASP

ASN

PHE

LEU

3

ASN

PRO

VAL

SER

ASP

ASN

PRO

ALA

TYR

4

GLU

VAL

LEU

GLN

HIS

VAL

LYS

ILE

PRO

THR

5

HIS

LEU

THR

ASP

VAL

TYR

GLU

GLN

6

THR

TRP

GLU

ALA

LEU

THR

ARG

LEU

ILE

7

PHE

VAL

GLY

SER

ASP

SER

LYS

GLY

ARG

8

GLN

TYR

PHE

TYR

GLY

LYS

MET

HIS

VAL

GLN

9

ASN

ARG

Samples:

sample_1: D2O, [U-2H], 10%; H2O 90%; sodium phosphate 50 mM; sodium chloride 150 mM; TopN, [U-100% 13C; U-100% 15N], 300 uM

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CCcoCNH TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMRView v9.2.0-b, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks