BMRB Entry 27112

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27112

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Title:
1H, 15N and 13C assignments of Drosophila brain-type fatty acid-binding protein ligated with oleic acid
Deposition date:
2017-05-25
Original release date:
2019-10-28
Authors:
Cheng, Yi-Yun; Lyu, Ping-Chiang
Citation:

Citation: Cheng, Yi-Yun; Huang, Yun-Fang; Lin, Hsin-Hui; Chang, Wun-Shaing Wayne; Lyu, Ping-Chiang. "The ligand-mediated affinity of brain-type fatty acid-binding protein for membranes determines the directionality of lipophilic cargo transport"  Biochim Biophys Acta Mol Cell Biol Lipids 1864, 158506-158506 (2019).
PubMed: 31404652

Assembly members:

Assembly members:
dFABP, polymer, 130 residues, Formula weight is not available
entity_OLA, non-polymer, 282.461 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dFABP: MSFVGKKYKLDKSENFDEYM KELGVGLVTRKMGNSLSPTV EVTLEGDTYTLTTTSTFKTS AISFKLGVEFDEETLDGRNV KSIITLDGNKLTQEQKGDKP TTIVREFTDNELITTLTIGN VKCVRVYKAV

Data sets:
Data typeCount
13C chemical shifts327
15N chemical shifts119
1H chemical shifts369

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dFABP1
2OLEIC ACID2

Entities:

Entity 1, dFABP 130 residues - Formula weight is not available

1   METSERPHEVALGLYLYSLYSTYRLYSLEU
2   ASPLYSSERGLUASNPHEASPGLUTYRMET
3   LYSGLULEUGLYVALGLYLEUVALTHRARG
4   LYSMETGLYASNSERLEUSERPROTHRVAL
5   GLUVALTHRLEUGLUGLYASPTHRTYRTHR
6   LEUTHRTHRTHRSERTHRPHELYSTHRSER
7   ALAILESERPHELYSLEUGLYVALGLUPHE
8   ASPGLUGLUTHRLEUASPGLYARGASNVAL
9   LYSSERILEILETHRLEUASPGLYASNLYS
10   LEUTHRGLNGLUGLNLYSGLYASPLYSPRO
11   THRTHRILEVALARGGLUPHETHRASPASN
12   GLULEUILETHRTHRLEUTHRILEGLYASN
13   VALLYSCYSVALARGVALTYRLYSALAVAL

Entity 2, OLEIC ACID - C18 H34 O2 - 282.461 Da.

1   OLA

Samples:

sample_1: dFABP, [U-100% 13C; U-100% 15N], 0.75 mM; potassium phosphate 50 mM; sodium azide 0.02%; D2O 10%; DSS na na; H2O 90%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D CB(CA)(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

PINE, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks