BMRB Entry 27108

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SH3 domain of Noxa1
Published: 2017-09-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27108

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for SH3 domain of Noxa1

Deposition date:

2017-05-18

Original release date:

2017-09-14

Authors:

Shrestha, Pravesh

Citation:

Citation: Shrestha, Pravesh; Yun, Ji-Hye; Ko, Yoon-Joo; Kim, Myeongkyu; Bae, Yun Soo; Lee, Weontae. "C-terminal tail of NADPH oxidase organizer 1 (Noxo1) mediates interaction with NADPH oxidase activator (Noxa1) in the NOX1 complex" Biochem. Biophys. Res. Commun. 490, 594-600 (2017).
PubMed: 28625920

Assembly members:

Assembly members:
NOX1_activator, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NOX1_activator: QRPVLYQVVAQHSYSAQGPE DLGFRQGDTVDVLCEVDQAW LEGHCDGRIGIFPKCVVPAG PRMS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	116
15N chemical shifts	57
1H chemical shifts	55

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NOX1 ativator	1

Entities:

Entity 1, NOX1 ativator 64 residues - Formula weight is not available

1

GLN

ARG

PRO

VAL

LEU

TYR

GLN

VAL

ALA

2

GLN

HIS

SER

TYR

SER

ALA

GLN

GLY

PRO

GLU

3

ASP

LEU

GLY

PHE

ARG

GLN

GLY

ASP

THR

VAL

4

ASP

VAL

LEU

CYS

GLU

VAL

ASP

GLN

ALA

TRP

5

LEU

GLU

GLY

HIS

CYS

ASP

GLY

ARG

ILE

GLY

6

ILE

PHE

PRO

LYS

CYS

VAL

PRO

ALA

GLY

7

PRO

ARG

MET

SER

Samples:

sample_1: NOX1 ativator, na, mM; HEPES 10 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.01%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DRX 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks