BMRB Entry 27104
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27104
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NMR-STAR v3 text file.
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Citation: de Vera, Ian Mitchelle; Zheng, Jie; Novick, Scott; Shang, Jinsai; Hughes, Travis; Brust, Richard; Munoz-Tello, Paola; Gardner, William; Marciano, David; Kong, Xiangming; Griffin, Patrick; Kojetin, Douglas. "Synergistic regulation of coregulator/nuclear receptor interaction by ligand and DNA" Structure ., 30253-30258 (2017).
PubMed: 28890360
Assembly members:
SRC-2_RID, polymer, 235 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-46
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 642 |
| 15N chemical shifts | 220 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SRC-2 RID | 1 |
Entities:
Entity 1, SRC-2 RID 235 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ASP | TYR | LYS | ASP | ASP | ASP | ASP | LYS | GLU | ||||
| 4 | ARG | ALA | ASP | GLY | GLN | SER | ARG | LEU | HIS | ASP | ||||
| 5 | SER | LYS | GLY | GLN | THR | LYS | LEU | LEU | GLN | LEU | ||||
| 6 | LEU | THR | THR | LYS | SER | ASP | GLN | MET | GLU | PRO | ||||
| 7 | SER | PRO | LEU | ALA | SER | SER | LEU | SER | ASP | THR | ||||
| 8 | ASN | LYS | ASP | SER | THR | GLY | SER | LEU | PRO | GLY | ||||
| 9 | SER | GLY | SER | THR | HIS | GLY | THR | SER | LEU | LYS | ||||
| 10 | GLU | LYS | HIS | LYS | ILE | LEU | HIS | ARG | LEU | LEU | ||||
| 11 | GLN | ASP | SER | SER | SER | PRO | VAL | ASP | LEU | ALA | ||||
| 12 | LYS | LEU | THR | ALA | GLU | ALA | THR | GLY | LYS | ASP | ||||
| 13 | LEU | SER | GLN | GLU | SER | SER | SER | THR | ALA | PRO | ||||
| 14 | GLY | SER | GLU | VAL | THR | ILE | LYS | GLN | GLU | PRO | ||||
| 15 | VAL | SER | PRO | LYS | LYS | LYS | GLU | ASN | ALA | LEU | ||||
| 16 | LEU | ARG | TYR | LEU | LEU | ASP | LYS | ASP | ASP | THR | ||||
| 17 | LYS | ASP | ILE | GLY | LEU | PRO | GLU | ILE | THR | PRO | ||||
| 18 | LYS | LEU | GLU | ARG | LEU | ASP | SER | LYS | THR | ASP | ||||
| 19 | PRO | ALA | SER | ASN | THR | LYS | LEU | ILE | ALA | MET | ||||
| 20 | LYS | THR | GLU | LYS | GLU | GLU | MET | SER | PHE | GLU | ||||
| 21 | PRO | GLY | ASP | GLN | PRO | GLY | SER | GLU | LEU | ASP | ||||
| 22 | ASN | LEU | GLU | GLU | ILE | LEU | ASP | ASP | LEU | GLN | ||||
| 23 | ASN | SER | GLN | LEU | PRO | GLN | LEU | PHE | PRO | ASP | ||||
| 24 | THR | ARG | PRO | GLY | ALA |
Samples:
sample_1: SRC-2 RID, [U-100% 13C; U-100% 15N], 400 uM; KPO4 20 mM; KCl 50 mM
sample_conditions_1: ionic strength: 70 mM; pH: 8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D (HaCa)CON | sample_1 | isotropic | sample_conditions_1 |
| 2D (HCaCO)N(CO)Ca | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CbCaCON | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CbCaNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CaNCaCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 700 MHz