BMRB Entry 27098

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27098

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Title:
1H, 15N and 13C resonance assignments of Mth10bTQQA
Deposition date:
2017-05-06
Original release date:
2017-09-15
Authors:
Xuan, Jinsong; Yao, Hongwei; Feng, Yingang; Wang, Jinfeng
Citation:

Citation: Xuan, Jinsong; Yao, Hongwei; Feng, Yingang; Wang, Jinfeng. "Backbone and side-chain (1)H, (15)N and (13)C resonance assignments of two Sac10b family members Mvo10b and Mth10bTQQA from archaea"  Biomol. NMR Assign. 11, 269-273 (2017).
PubMed: 28812261

Assembly members:

Assembly members:
mth10btqqa, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Methanothermococcus thermolithotrophicus   Taxonomy ID: 2186   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanothermococcus thermolithotrophicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mth10btqqa: MDSTVYVGNKGVTNYVQAVQ TQFNTENAKEVRIKARGKAI SRAVDVEEMVRNRFLAEVKV KEINLGTDKVQNDDGKSVNI STIEIVLEKLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts106
1H chemical shifts673

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mth10btqqa, chain 11
2mth10btqqa, chain 21

Entities:

Entity 1, mth10btqqa, chain 1 97 residues - Formula weight is not available

1   METASPSERTHRVALTYRVALGLYASNLYS
2   GLYVALTHRASNTYRVALGLNALAVALGLN
3   THRGLNPHEASNTHRGLUASNALALYSGLU
4   VALARGILELYSALAARGGLYLYSALAILE
5   SERARGALAVALASPVALGLUGLUMETVAL
6   ARGASNARGPHELEUALAGLUVALLYSVAL
7   LYSGLUILEASNLEUGLYTHRASPLYSVAL
8   GLNASNASPASPGLYLYSSERVALASNILE
9   SERTHRILEGLUILEVALLEUGLULYSLEU
10   GLUHISHISHISHISHISHIS

Samples:

sample_n15: mth10btqqa, [U-15N], 1 mM; potassium phosphate 50 mM; potassium chloride 100 mM; sodium azide 0.01 mM; DSS 0.01%

sample_c13: mth10btqqa, [U-13C], 1 mM; potassium phosphate 50 mM; potassium chloride 100 mM; sodium azide 0.01 mM; DSS 0.01%

sample_c13n15: mth10btqqa, [U-13C; U-15N], 1 mM; potassium phosphate 50 mM; potassium chloride 100 mM; sodium azide 0.01 mM; DSS 0.01%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_n15isotropicsample_conditions_1
3D 1H-15N NOESYsample_n15isotropicsample_conditions_1
2D 1H-13C HSQCsample_c13isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_c13isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_c13isotropicsample_conditions_1
3D CBCA(CO)NHsample_c13n15isotropicsample_conditions_1
3D HNCACBsample_c13n15isotropicsample_conditions_1
3D HNCAsample_c13n15isotropicsample_conditions_1
3D HNCOsample_c13n15isotropicsample_conditions_1
3D HN(CA)COsample_c13n15isotropicsample_conditions_1
3D HBHA(CO)NHsample_c13n15isotropicsample_conditions_1
3D HBHANHsample_c13n15isotropicsample_conditions_1
3D HCCH-TOCSYsample_c13n15isotropicsample_conditions_1
3D HCCH-COSYsample_c13n15isotropicsample_conditions_1
3D CCH-TOCSYsample_c13n15isotropicsample_conditions_1
3D CCH-COSYsample_c13n15isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

Felix, Accelrys Software Inc. - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

UNP A5JSW7
AlphaFold A5JSW7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks