BMRB Entry 27096

Name: apo-AFABP chemical shift assignments
Published: 2020-01-06

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27096

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

apo-AFABP chemical shift assignments

Deposition date:

2017-05-04

Original release date:

2020-01-06

Authors:

Wang, Qian; Bernard, Cedric; Stark, Ruth

Citation:

Citation: Wang, Qian; Rizk, Samar; Bernard, Cedric; Lai, MayPoh; Kam, David; Storch, Judith; Stark, Ruth. "Protocols and pitfalls in obtaining fatty acid-binding proteins for biophysical studies of ligand-protein and protein-protein interactions" Biochem. Biophys. Rep. 10, 318-324 (2017).
PubMed: 28955759

Assembly members:

Assembly members:
AFABP_mouse, polymer, 133 residues, 14706.9 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AFABP_mouse: GMCDAFVGTWKLVSSENFDD YMKEVGVGFATRKVAGMAKP NMIISVNGDLVTIRSESTFK NTEISFKLGVEFDEITADDR KVKSIITLDGGALVQVQKWD GKSTTIKRKRDGDKLVVECV MKGVTSTRVYERA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- spectral_peak_list_1
- spectral_peak_list_2

Data type	Count
15N chemical shifts	123
1H chemical shifts	390

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apo_AFABP	1

Entities:

Entity 1, apo_AFABP 133 residues - 14706.9 Da.

TEV cleavage left the glycine at the N-termus. Assignment table starts from 1,C.

1

GLY

MET

CYS

ASP

ALA

PHE

VAL

GLY

THR

TRP

2

LYS

LEU

VAL

SER

GLU

ASN

PHE

ASP

3

TYR

MET

LYS

GLU

VAL

GLY

VAL

GLY

PHE

ALA

4

THR

ARG

LYS

VAL

ALA

GLY

MET

ALA

LYS

PRO

5

ASN

MET

ILE

SER

VAL

ASN

GLY

ASP

LEU

6

VAL

THR

ILE

ARG

SER

GLU

SER

THR

PHE

LYS

7

ASN

THR

GLU

ILE

SER

PHE

LYS

LEU

GLY

VAL

8

GLU

PHE

ASP

GLU

ILE

THR

ALA

ASP

ARG

9

LYS

VAL

LYS

SER

ILE

THR

LEU

ASP

GLY

10

GLY

ALA

LEU

VAL

GLN

VAL

GLN

LYS

TRP

ASP

11

GLY

LYS

SER

THR

ILE

LYS

ARG

LYS

ARG

12

ASP

GLY

ASP

LYS

LEU

VAL

GLU

CYS

VAL

13

MET

LYS

GLY

VAL

THR

SER

THR

ARG

VAL

TYR

14

GLU

ARG

ALA

Samples:

sample_1: AFABP mouse, [U-98% 15N], 710 uM; H2O 90 % v/v; potassium chloride 150 mM; potassium phosphate 10 mM; sodium azide 0.2 g/L; D2O, [U-99% 2H], 10 % v/v

sample_conditions_1: ionic strength: 0.17552 M; pH: 7.4; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRView vv9.1.0-b10, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, data analysis, peak picking

NMR spectrometers:

Bruker Avance 500 MHz