BMRB Entry 27092

Name: Chemical Shifts of Transmembrane Segment XI of the Na+/H+ Antiporter of S. pombe in DPC
Published: 2017-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27092

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shifts of Transmembrane Segment XI of the Na+/H+ Antiporter of S. pombe in DPC

Deposition date:

2017-05-03

Original release date:

2017-11-10

Authors:

Shin, Kyungsoo; Fligel, Larry; Rainey, Jan

Citation:

Citation: Dutta, Debajyoti; Shin, Kyungsoo; Rainey, Jan; Fliegel, Larry. "Transmembrane Segment XI of the Na(+)/H(+) Antiporter of S. pombe is a Critical Part of the Ion Translocation Pore." Sci. Rep. 7, 12793-12793 (2017).
PubMed: 29038548

Assembly members:

Assembly members:
Sod2_TMXI, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sod2_TMXI: XKKKEALFVGHFGPIGVCAV YMAFLAKLLLKKKX

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	88
1H chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sod2_TMXI	1

Entities:

Entity 1, Sod2_TMXI 34 residues - Formula weight is not available

N-terminal Acetylation C-terminal Amide cap

1

ACE

LYS

GLU

ALA

LEU

PHE

VAL

GLY

2

HIS

PHE

GLY

PRO

ILE

GLY

VAL

CYS

ALA

VAL

3

TYR

MET

ALA

PHE

LEU

ALA

LYS

LEU

4

LYS

NH2

Samples:

sample_1: Sod2_TMXI 1 mM; DPC-d38, [U-99% 2H], 150 mM; DSS 1 mM; sodium azide 1 mM; CD3COO, [U-99% 2H], 20 mM; DTT-d6, [U-99% 2H], 10 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 308.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNMR v2.4, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 700 MHz