BMRB Entry 27091

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27091

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for EarP
Deposition date:
2017-05-03
Original release date:
2017-10-03
Authors:
Macosek, Jakub; Hennig, Janosch
Citation:

Citation: Krafczyk, Ralph; Macosek, Jakub; Jagtap, Pravin Kumar Ankush; Gast, Daniel; Wunder, Swetlana; Mitra, Prithiba; Jha, Amit Kumar; Rohr, Jurgen; Hoffmann-Roder, Anja; Jung, Kirsten; Hennig, Janosch; Lassak, Jurgen. "Structural Basis for EarP-Mediated Arginine Glycosylation of Translation Elongation Factor EF-P"  MBio 8, e01412-e01417 (2017).
PubMed: 28951478

Assembly members:

Assembly members:
EarP, polymer, 385 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas putida   Taxonomy ID: 303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAD33

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EarP: MKATWDIFCSVVDNYGDIGV TWRLARQLVAEHGLAVRLWV DDLNAFTPMCPGADATAAQQ WQHGVDVRHWPAAWLPVAPA DVVIGAFACQLPAAYVEAMR ARPQPPLWLNLEYLSAEDWV EGCHGLPSPQPNGLRKVFFF PGFTDKTGGLLREGSLLARR DGFQQSAEARRAFLQGLGVD LVPGALLISLFAYENPQLGN WLDALATADQPCHLLVPQGR VVAGLSQWLGEGPLHVGDVR TRGALTVQVLPFVSQDDFDR LLWSCDFNAVRGEDSFVRAQ WAGQPMLWHIYVQDENAHWE KLEAFLAHYRCGLSDDADAA LLGLWRAWNMDFDMGQAWRA ARQHWPELQQHARLWGARQA AQPDLATALVHFYRNSLGSH HHHHH

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts237
1H chemical shifts236

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EarP1

Entities:

Entity 1, EarP 385 residues - Formula weight is not available

1   METLYSALATHRTRPASPILEPHECYSSER
2   VALVALASPASNTYRGLYASPILEGLYVAL
3   THRTRPARGLEUALAARGGLNLEUVALALA
4   GLUHISGLYLEUALAVALARGLEUTRPVAL
5   ASPASPLEUASNALAPHETHRPROMETCYS
6   PROGLYALAASPALATHRALAALAGLNGLN
7   TRPGLNHISGLYVALASPVALARGHISTRP
8   PROALAALATRPLEUPROVALALAPROALA
9   ASPVALVALILEGLYALAPHEALACYSGLN
10   LEUPROALAALATYRVALGLUALAMETARG
11   ALAARGPROGLNPROPROLEUTRPLEUASN
12   LEUGLUTYRLEUSERALAGLUASPTRPVAL
13   GLUGLYCYSHISGLYLEUPROSERPROGLN
14   PROASNGLYLEUARGLYSVALPHEPHEPHE
15   PROGLYPHETHRASPLYSTHRGLYGLYLEU
16   LEUARGGLUGLYSERLEULEUALAARGARG
17   ASPGLYPHEGLNGLNSERALAGLUALAARG
18   ARGALAPHELEUGLNGLYLEUGLYVALASP
19   LEUVALPROGLYALALEULEUILESERLEU
20   PHEALATYRGLUASNPROGLNLEUGLYASN
21   TRPLEUASPALALEUALATHRALAASPGLN
22   PROCYSHISLEULEUVALPROGLNGLYARG
23   VALVALALAGLYLEUSERGLNTRPLEUGLY
24   GLUGLYPROLEUHISVALGLYASPVALARG
25   THRARGGLYALALEUTHRVALGLNVALLEU
26   PROPHEVALSERGLNASPASPPHEASPARG
27   LEULEUTRPSERCYSASPPHEASNALAVAL
28   ARGGLYGLUASPSERPHEVALARGALAGLN
29   TRPALAGLYGLNPROMETLEUTRPHISILE
30   TYRVALGLNASPGLUASNALAHISTRPGLU
31   LYSLEUGLUALAPHELEUALAHISTYRARG
32   CYSGLYLEUSERASPASPALAASPALAALA
33   LEULEUGLYLEUTRPARGALATRPASNMET
34   ASPPHEASPMETGLYGLNALATRPARGALA
35   ALAARGGLNHISTRPPROGLULEUGLNGLN
36   HISALAARGLEUTRPGLYALAARGGLNALA
37   ALAGLNPROASPLEUALATHRALALEUVAL
38   HISPHETYRARGASNSERLEUGLYSERHIS
39   HISHISHISHISHIS

Samples:

sample_1: EarP, [U-100% 13C; U-100% 15N; U-80% 2H], 0.375 mM; sodium phosphate 0.1 mM

sample_conditions_1: pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - data analysis

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP Q88LS1
AlphaFold Q88LS1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks