BMRB Entry 27089

Name: ISCU(D39V)
Published: 2017-05-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27089

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ISCU(D39V)

Deposition date:

2017-04-30

Original release date:

2017-05-23

Authors:

Cai, Kai; Markley, John

Citation:

Citation: Cai, Kai; Frederick, Ronnie; Tonelli, Marco; Markley, John. "ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin" Biochemistry 57, 1491-1500 (2018).
PubMed: 29406711

Assembly members:

Assembly members:
ISCU, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ISCU: RLYHKKVVDHYENPRNVGSL DKTSKNVGTGLVGAPACGVV MKLQIQVDEKGKIVDARFKT FGCGSAIASSSLATEWVKGK TVEEALTIKNTDIAKELCLP PVKLHCSMLAEDAIKAALAD YKLKQEPKKGEAEKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- spectral_peak_list_1
- spectral_peak_list_2

Data type	Count
13C chemical shifts	211
15N chemical shifts	103
1H chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ISCU monomer	1

Entities:

Entity 1, ISCU monomer 135 residues - Formula weight is not available

1

ARG

LEU

TYR

HIS

LYS

VAL

ASP

HIS

2

TYR

GLU

ASN

PRO

ARG

ASN

VAL

GLY

SER

LEU

3

ASP

LYS

THR

SER

LYS

ASN

VAL

GLY

THR

GLY

4

LEU

VAL

GLY

ALA

PRO

ALA

CYS

GLY

VAL

5

MET

LYS

LEU

GLN

ILE

GLN

VAL

ASP

GLU

LYS

6

GLY

LYS

ILE

VAL

ASP

ALA

ARG

PHE

LYS

THR

7

PHE

GLY

CYS

GLY

SER

ALA

ILE

ALA

SER

8

SER

LEU

ALA

THR

GLU

TRP

VAL

LYS

GLY

LYS

9

THR

VAL

GLU

ALA

LEU

THR

ILE

LYS

ASN

10

THR

ASP

ILE

ALA

LYS

GLU

LEU

CYS

LEU

PRO

11

PRO

VAL

LYS

LEU

HIS

CYS

SER

MET

LEU

ALA

12

GLU

ASP

ALA

ILE

LYS

ALA

LEU

ALA

ASP

13

TYR

LYS

LEU

LYS

GLN

GLU

PRO

LYS

GLY

14

GLU

ALA

GLU

LYS

Samples:

sample_1: ISCU(D39V), [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 20 mM; sodium chloride 150 mM; TCEP 3 mM; DSS 0.01 mM; EDTA 5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.6; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks