BMRB Entry 27085

Name: 1H, 13C, 15N backbone NMR assignment of N-terminal domain of SaHPF
Published: 2017-10-05

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PDB ID: 6qbz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27085
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N backbone NMR assignment of N-terminal domain of SaHPF

Deposition date:

2017-04-27

Original release date:

2017-10-05

Authors:

Usachev, Konstantin; Ayupov, Rustam; Validov, Shamil; Khusainov, Iskander; Yusupov, Marat

Citation:

Citation: Usachev, Konstantin; Ayupov, Rustam; Validov, Shamil; Khusainov, Iskander; Yusupov, Marat. "NMR assignments of the N-terminal domain of Staphylococcus aureus hibernation promoting factor (SaHPF)" Biomol. NMR Assignments 12, 85-89 (2018).
PubMed: 28980143

Assembly members:

Assembly members:
SaHPF_NTD, polymer, 110 residues, 12913.7078 Da.

Natural source:

Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGS21A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SaHPF_NTD: MIRFEIHGDNLTITDAIRNY IEEKIGKLERYFNDVPNAVA HVKVKTYSNSATKIEVTIPL KNVTLRAEERNDDLYAGIDL INNKLERQVRKYKTRINRKS RDRGDQEVFV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
spectral_peak_list

Data type	Count
13C chemical shifts	435
15N chemical shifts	102
1H chemical shifts	501

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SaHPF NTD	1

Entities:

Entity 1, SaHPF NTD 110 residues - 12913.7078 Da.

1	MET	ILE	ARG	PHE	GLU	ILE	HIS	GLY	ASP	ASN
2	LEU	THR	ILE	THR	ASP	ALA	ILE	ARG	ASN	TYR
3	ILE	GLU	GLU	LYS	ILE	GLY	LYS	LEU	GLU	ARG
4	TYR	PHE	ASN	ASP	VAL	PRO	ASN	ALA	VAL	ALA
5	HIS	VAL	LYS	VAL	LYS	THR	TYR	SER	ASN	SER
6	ALA	THR	LYS	ILE	GLU	VAL	THR	ILE	PRO	LEU
7	LYS	ASN	VAL	THR	LEU	ARG	ALA	GLU	GLU	ARG
8	ASN	ASP	ASP	LEU	TYR	ALA	GLY	ILE	ASP	LEU
9	ILE	ASN	ASN	LYS	LEU	GLU	ARG	GLN	VAL	ARG
10	LYS	TYR	LYS	THR	ARG	ILE	ASN	ARG	LYS	SER
11	ARG	ASP	ARG	GLY	ASP	GLN	GLU	VAL	PHE	VAL

Samples:

NTD-SaHPF: SaHPF NTD, [U-100% 13C; U-100% 15N], 0.62 mM; NH4Cl 200.00 mM

condition1: ionic strength: 0.200 M; pH: 7.600; pressure: 1.000 atm; temperature: 308.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	NTD-SaHPF	isotropic	condition1
2D 1H-13C HSQC/HMQC	NTD-SaHPF	isotropic	condition1
2D 1H-13C HSQC/HMQC	NTD-SaHPF	isotropic	condition1
3D HNCA	NTD-SaHPF	isotropic	condition1
3D CBCA(CO)NH	NTD-SaHPF	isotropic	condition1
3D HNCACB	NTD-SaHPF	isotropic	condition1
3D HNCO	NTD-SaHPF	isotropic	condition1
HN(CA)CO (H[N[ca[CO]]])	NTD-SaHPF	isotropic	condition1
CC(CO)NH (C_coNH.relayed)	NTD-SaHPF	isotropic	condition1
3D H(CCO)NH	NTD-SaHPF	isotropic	condition1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	ILE	ARG	PHE	GLU	ILE	HIS	GLY	ASP	ASN
2	LEU	THR	ILE	THR	ASP	ALA	ILE	ARG	ASN	TYR
3	ILE	GLU	GLU	LYS	ILE	GLY	LYS	LEU	GLU	ARG
4	TYR	PHE	ASN	ASP	VAL	PRO	ASN	ALA	VAL	ALA
5	HIS	VAL	LYS	VAL	LYS	THR	TYR	SER	ASN	SER
6	ALA	THR	LYS	ILE	GLU	VAL	THR	ILE	PRO	LEU
7	LYS	ASN	VAL	THR	LEU	ARG	ALA	GLU	GLU	ARG
8	ASN	ASP	ASP	LEU	TYR	ALA	GLY	ILE	ASP	LEU
9	ILE	ASN	ASN	LYS	LEU	GLU	ARG	GLN	VAL	ARG
10	LYS	TYR	LYS	THR	ARG	ILE	ASN	ARG	LYS	SER
11	ARG	ASP	ARG	GLY	ASP	GLN	GLU	VAL	PHE	VAL

1	MET	ILE	ARG	PHE	GLU	ILE	HIS	GLY	ASP	ASN
2	LEU	THR	ILE	THR	ASP	ALA	ILE	ARG	ASN	TYR
3	ILE	GLU	GLU	LYS	ILE	GLY	LYS	LEU	GLU	ARG
4	TYR	PHE	ASN	ASP	VAL	PRO	ASN	ALA	VAL	ALA
5	HIS	VAL	LYS	VAL	LYS	THR	TYR	SER	ASN	SER
6	ALA	THR	LYS	ILE	GLU	VAL	THR	ILE	PRO	LEU
7	LYS	ASN	VAL	THR	LEU	ARG	ALA	GLU	GLU	ARG
8	ASN	ASP	ASP	LEU	TYR	ALA	GLY	ILE	ASP	LEU
9	ILE	ASN	ASN	LYS	LEU	GLU	ARG	GLN	VAL	ARG
10	LYS	TYR	LYS	THR	ARG	ILE	ASN	ARG	LYS	SER
11	ARG	ASP	ARG	GLY	ASP	GLN	GLU	VAL	PHE	VAL

1	MET	ILE	ARG	PHE	GLU	ILE	HIS	GLY	ASP	ASN
2	LEU	THR	ILE	THR	ASP	ALA	ILE	ARG	ASN	TYR
3	ILE	GLU	GLU	LYS	ILE	GLY	LYS	LEU	GLU	ARG
4	TYR	PHE	ASN	ASP	VAL	PRO	ASN	ALA	VAL	ALA
5	HIS	VAL	LYS	VAL	LYS	THR	TYR	SER	ASN	SER
6	ALA	THR	LYS	ILE	GLU	VAL	THR	ILE	PRO	LEU
7	LYS	ASN	VAL	THR	LEU	ARG	ALA	GLU	GLU	ARG
8	ASN	ASP	ASP	LEU	TYR	ALA	GLY	ILE	ASP	LEU
9	ILE	ASN	ASN	LYS	LEU	GLU	ARG	GLN	VAL	ARG
10	LYS	TYR	LYS	THR	ARG	ILE	ASN	ARG	LYS	SER
11	ARG	ASP	ARG	GLY	ASP	GLN	GLU	VAL	PHE	VAL