BMRB Entry 27081

Name: 1H, 13C, and 15N backbone and side chain resonance assignments for a structured domain in atg32
Published: 2017-11-16

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PDB ID: 5wlp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27081
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N backbone and side chain resonance assignments for a structured domain in atg32

Deposition date:

2017-04-22

Original release date:

2017-11-16

Authors:

Xia, Xue; Pellegrini, Maria; Ragusa, Michael

Citation:

Citation: Xia, Xue; Pellegrini, Maria; Ragusa, Michael. "Backbone and side chain resonance assignments for a structured domain within Atg32" Biomol. NMR Assignments 11, 211-214 (2017).
PubMed: 28766175

Assembly members:

Assembly members:
atg32, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 TEV LIC cloning vector (1B)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
atg32: SNATNSFVMPKLSLTQKNPV FRLLILGRTGSSFYQSIPKE YQSLFELPKYHDSATFPQYT GIVIIFQELREMVSLLNRIV QYSQGKPVIPIcQPGQVIQV KNVLKSFLRNKLVKLLFPPV VVTNKRDLKKMFQRLQDLSL EYGED

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	603
15N chemical shifts	140
1H chemical shifts	1040

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	atg32	1

Entities:

Entity 1, atg32 145 residues - Formula weight is not available

1

SER

ASN

ALA

THR

ASN

SER

PHE

VAL

MET

PRO

2

LYS

LEU

SER

LEU

THR

GLN

LYS

ASN

PRO

VAL

3

PHE

ARG

LEU

ILE

LEU

GLY

ARG

THR

GLY

4

SER

PHE

TYR

GLN

SER

ILE

PRO

LYS

GLU

5

TYR

GLN

SER

LEU

PHE

GLU

LEU

PRO

LYS

TYR

6

HIS

ASP

SER

ALA

THR

PHE

PRO

GLN

TYR

THR

7

GLY

ILE

VAL

ILE

PHE

GLN

GLU

LEU

ARG

8

GLU

MET

VAL

SER

LEU

ASN

ARG

ILE

VAL

9

GLN

TYR

SER

GLN

GLY

LYS

PRO

VAL

ILE

PRO

10

ILE

CYS

GLN

PRO

GLY

GLN

VAL

ILE

GLN

VAL

11

LYS

ASN

VAL

LEU

LYS

SER

PHE

LEU

ARG

ASN

12

LYS

LEU

VAL

LYS

LEU

PHE

PRO

VAL

13

VAL

THR

ASN

LYS

ARG

ASP

LEU

LYS

14

MET

PHE

GLN

ARG

LEU

GLN

ASP

LEU

SER

LEU

15

GLU

TYR

GLY

GLU

ASP

Samples:

sample_1: atg32, [U-99% 13C; U-99% 15N], 750 uM; sodium phosphate 20 mM; sodium chloride 100 mM; TCEP 0.2 mM

sample_2: atg32 370 uM; sodium phosphate 20 mM; sodium chloride 100 mM; TCEP 0.2 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Ascend 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks