BMRB Entry 27065

Name: 13C, 15N chemical shift assignments of MAK33 VL S20N amyloid fibrils
Published: 2017-04-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27065

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C, 15N chemical shift assignments of MAK33 VL S20N amyloid fibrils

Deposition date:

2017-04-06

Original release date:

2017-04-27

Authors:

Hora, Manuel; Sarkar, Riddhiman; Morris, Vanessa; Xue, Kai; Prade, Elke; Harding, Emma; Buchner, Johannes; Reif, Bernd

Citation:

Citation: Hora, Manuel; Sarkar, Riddhiman; Morris, Vanessa; Xue, Kai; Prade, Elke; Harding, Emma; Buchner, Johannes; Reif, Bernd. "MAK33 antibody light chain amyloid fibrils are similar to oligomeric precursors." PLoS ONE 12, e0181799-e0181799 (2017).
PubMed: 28746363

Assembly members:

Assembly members:
MAK33_VL, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MAK33_VL: DIVLTQSPATLSVTPGDSVN LSCRASQSISNNLHWYQQKS HESPRLLIKYASQSISGIPS RFSGSGSGTDFTLSINSVET EDFGMYFCQQSNSWPLTFGA GTKLELKR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	111
15N chemical shifts	34

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MAK33 VL amyloid fibril	1

Entities:

Entity 1, MAK33 VL amyloid fibril 108 residues - Formula weight is not available

this is the variable domain of an antibody light chain

1

ASP

ILE

VAL

LEU

THR

GLN

SER

PRO

ALA

THR

2

LEU

SER

VAL

THR

PRO

GLY

ASP

SER

VAL

ASN

3

LEU

SER

CYS

ARG

ALA

SER

GLN

SER

ILE

SER

4

ASN

LEU

HIS

TRP

TYR

GLN

LYS

SER

5

HIS

GLU

SER

PRO

ARG

LEU

ILE

LYS

TYR

6

ALA

SER

GLN

SER

ILE

SER

GLY

ILE

PRO

SER

7

ARG

PHE

SER

GLY

SER

GLY

SER

GLY

THR

ASP

8

PHE

THR

LEU

SER

ILE

ASN

SER

VAL

GLU

THR

9

GLU

ASP

PHE

GLY

MET

TYR

PHE

CYS

GLN

10

SER

ASN

SER

TRP

PRO

LEU

THR

PHE

GLY

ALA

11

GLY

THR

LYS

LEU

GLU

LEU

LYS

ARG

Samples:

sample_1: MAK33 VL, [U-13C; U-15N], mM; acetic acid 25 mM; phosphoric acid 25 mM; potassium chloride 50 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 175 mM; pH: 2; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D PDSD (50 ms)	sample_1	solid	sample_conditions_1
2D NCACX	sample_1	solid	sample_conditions_1
3D NCACX	sample_1	solid	sample_conditions_1
2D NCOCX	sample_1	solid	sample_conditions_1
3D NCOCX	sample_1	solid	sample_conditions_1
2D NCACB	sample_1	solid	sample_conditions_1
3D NCACB	sample_1	solid	sample_conditions_1
2D NcoCACB	sample_1	solid	sample_conditions_1
3D NCOCA	sample_1	solid	sample_conditions_1
3D CANCO	sample_1	solid	sample_conditions_1
2D 15N INEPT	sample_1	solid	sample_conditions_1

Software:

CcpNMR_Analysis v2, CCPN - data analysis

NMR spectrometers:

Bruker Avance 750 MHz