BMRB Entry 27059

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27059

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Title:
15N, 13C assignments of Leptosphaeria Rhodopsin
Deposition date:
2017-03-29
Original release date:
2021-07-22
Authors:
Liu, Jing; Liu, Chang; Wang, Shenlin
Citation:

Citation: Liu, Chang; Liu, Jing; Xu, Xiaojun; Xiang, ShengQi; Wang, Shenlin. "Gd 3+-chelated lipid accelerates solid-state NMR spectroscopy of seven-transmembrane proteins"  J. Biomol. NMR 68, 203-214 (2017).
PubMed: 28560567

Assembly members:

Assembly members:
LR, polymer, 313 residues, Formula weight is not available
entity_RET, non-polymer, 284.436 Da.

Natural source:

Natural source:   Common Name: blackleg of rapeseed fungus   Taxonomy ID: 5022   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Leptosphaeria maculans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZaA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LR: MIVDQFEEVLMKTSQLFPLP TATQSAQPTHVAPVPTVLPD TPIYETVGDSGSKTLWVVFV LMLIASAAFTALSWKIPVNR RLYHVITTIITLTAALSYFA MATGHGVALNKIVIRTQHDH VPDTYETVYRQVYYARYIDW AITTPLLLLDLGLLAGMSGA HIFMAIVADLIMVLTGLFAA FGSEGTPQKWGWYTIACIAY IFVVWHLVLNGGANARVKGE KLRSFFVAIGAYTLILWTAY PIVWGLADGARKIGVDGEII AYAVLDVLAKGVFGAWLLVT HANLRESDVELNGFWANGLN REGAIRIGEDDGA

Data sets:
Data typeCount
13C chemical shifts566
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LR-retinal1
2retinal2

Entities:

Entity 1, LR-retinal 313 residues - Formula weight is not available

1   METILEVALASPGLNPHEGLUGLUVALLEU
2   METLYSTHRSERGLNLEUPHEPROLEUPRO
3   THRALATHRGLNSERALAGLNPROTHRHIS
4   VALALAPROVALPROTHRVALLEUPROASP
5   THRPROILETYRGLUTHRVALGLYASPSER
6   GLYSERLYSTHRLEUTRPVALVALPHEVAL
7   LEUMETLEUILEALASERALAALAPHETHR
8   ALALEUSERTRPLYSILEPROVALASNARG
9   ARGLEUTYRHISVALILETHRTHRILEILE
10   THRLEUTHRALAALALEUSERTYRPHEALA
11   METALATHRGLYHISGLYVALALALEUASN
12   LYSILEVALILEARGTHRGLNHISASPHIS
13   VALPROASPTHRTYRGLUTHRVALTYRARG
14   GLNVALTYRTYRALAARGTYRILEASPTRP
15   ALAILETHRTHRPROLEULEULEULEUASP
16   LEUGLYLEULEUALAGLYMETSERGLYALA
17   HISILEPHEMETALAILEVALALAASPLEU
18   ILEMETVALLEUTHRGLYLEUPHEALAALA
19   PHEGLYSERGLUGLYTHRPROGLNLYSTRP
20   GLYTRPTYRTHRILEALACYSILEALATYR
21   ILEPHEVALVALTRPHISLEUVALLEUASN
22   GLYGLYALAASNALAARGVALLYSGLYGLU
23   LYSLEUARGSERPHEPHEVALALAILEGLY
24   ALATYRTHRLEUILELEUTRPTHRALATYR
25   PROILEVALTRPGLYLEUALAASPGLYALA
26   ARGLYSILEGLYVALASPGLYGLUILEILE
27   ALATYRALAVALLEUASPVALLEUALALYS
28   GLYVALPHEGLYALATRPLEULEUVALTHR
29   HISALAASNLEUARGGLUSERASPVALGLU
30   LEUASNGLYPHETRPALAASNGLYLEUASN
31   ARGGLUGLYALAILEARGILEGLYGLUASP
32   ASPGLYALA

Entity 2, retinal - C20 H28 O - 284.436 Da.

1   RET

Samples:

sample_1: LR, [U-100% 13C; U-100% 15N], 2.5:1 w/w

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
NCAsample_1isotropicsample_conditions_1
NCOsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANCOCXsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz