BMRB Entry 27057

Name: Chemical Shift Assignment of the PP1 and Microtubule binding domain of human KNL-1
Published: 2019-01-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27057

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignment of the PP1 and Microtubule binding domain of human KNL-1

Deposition date:

2017-03-24

Original release date:

2019-01-10

Authors:

Bajaj, Rakhi; Page, Rebecca; Peti, Wolfgang

Citation:

Citation: Bajaj, Rakhi; Bollen, Mathieu; Peti, Wolfgang; Page, Rebecca. "KNL1 Binding to PP1 and Microtubules Is Mutually Exclusive." Structure 26, 1327-11336 (2018).
PubMed: 30100357

Assembly members:

Assembly members:
KNL-1, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM30-MBP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KNL-1: GAMGMDGVSSEANEENDNIE RPVRRRHSSILKPPRSPLQD LRGGNETVQESNALRNKKNS RRVSFADTIKVFQTESHMKI VRKS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	232
15N chemical shifts	78
1H chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human Kinetochore null protein 1	1

Entities:

Entity 1, Human Kinetochore null protein 1 84 residues - Formula weight is not available

Residues 1-4 represents a cloning site artifact. Residues 5-84 represents the N-terminal 80 residues in KNL-1

1

GLY

ALA

MET

GLY

MET

ASP

GLY

VAL

SER

2

GLU

ALA

ASN

GLU

ASN

ASP

ASN

ILE

GLU

3

ARG

PRO

VAL

ARG

HIS

SER

ILE

4

LEU

LYS

PRO

ARG

SER

PRO

LEU

GLN

ASP

5

LEU

ARG

GLY

ASN

GLU

THR

VAL

GLN

GLU

6

SER

ASN

ALA

LEU

ARG

ASN

LYS

ASN

SER

7

ARG

VAL

SER

PHE

ALA

ASP

THR

ILE

LYS

8

VAL

PHE

GLN

THR

GLU

SER

HIS

MET

LYS

ILE

9

VAL

ARG

LYS

SER

Samples:

sample_1: KNL-1, [U-98% 13C; U-98% 15N], 0.6 mM; HEPES 10 mM; Sodium cloride 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.8.4.2, Keller and Wuthrich - chemical shift assignment

Topspin_3.1 v3.5, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 850 MHz
Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks