BMRB Entry 27056

Title:
C. elegans SAS-5 N-term backbone assignments   PubMed: 29367435
Deposition date:
2017-03-24
Original release date:
2018-02-02
Authors:
Rogala, Kacper; Vakonakis, Ioannis
Citation:

Citation: Bianchi, Sarah; Rogala, Kacper; Dynes, Nicola; Hilbert, Manuel; Leidel, Sebastian; Steinmetz, Michel; Gonczy, Pierre; Vakonakis, Ioannis. "Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly"  Mol. Biol. Cell 29, 722-735 (2018).

Assembly members:

Assembly members:
sas-5N, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFLOAT

Experimental source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFLOAT

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts107
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sas-5N1

Entities:

Entity 1, sas-5N 123 residues - Formula weight is not available

Residues 1 and 2 are cloning artifacts. Numbering in assignments corresponds to uniprot Q20010 except for the cloning artefact.

1   GLYPROASNASNTYRASPASPLEUPROCYS
2   SERILETYRPHELYSLYSPROTHRVALGLN
3   GLUPHEVALASPGLNPROARGVALPHEGLU
4   ASPSERGLUVALPROALAPHEGLNGLUVAL
5   LEUGLNLEUPROGLNGLUARGTHRLYSPRO
6   PROVALPROSERTHRGLNPROILEVALALA
7   ALAVALGLUVALALALYSLYSLYSSERCYS
8   LEUSERALAPROLYSPROARGLYSGLUPRO
9   PROSERHISPROALALEUARGGLNLYSTHR
10   VALALAPHEGLYLYSTHRVALASNVALSER
11   GLNTHRVALGLUGLYTHRSERARGASNSER
12   LYSLYSVALLEUALASERTHRMETSERALA
13   GLNASNTHR

Samples:

sample_1: sas-5N, [U-100% 13C; U-100% 15N], 0.5 mM; Potassium PIPES (K-PIPES) 80 mM; EGTA 1 mM; MgCl2 1 mM; NaN3 0.02 % w/v; DSS 50 uM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAHBsample_1isotropicsample_conditions_1

Software:

Omega_spectrometer_software vvBeta 6.0.3b2, GE - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis, CCPN - data analysis

NMR spectrometers:

  • Home-built OMEGA 750 MHz

Related Database Links:

UNP Q20010

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts