BMRB Entry 27056

Name: C. elegans SAS-5 N-term backbone assignments
Published: 2018-02-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27056

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

C. elegans SAS-5 N-term backbone assignments

Deposition date:

2017-03-24

Original release date:

2018-02-02

Authors:

Rogala, Kacper; Vakonakis, Ioannis

Citation:

Citation: Bianchi, Sarah; Rogala, Kacper; Dynes, Nicola; Hilbert, Manuel; Leidel, Sebastian; Steinmetz, Michel; Gonczy, Pierre; Vakonakis, Ioannis. "Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly" Mol. Biol. Cell 29, 722-735 (2018).
PubMed: 29367435

Assembly members:

Assembly members:
sas-5N, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pFLOAT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sas-5N: GPNNYDDLPCSIYFKKPTVQ EFVDQPRVFEDSEVPAFQEV LQLPQERTKPPVPSTQPIVA AVEVAKKKSCLSAPKPRKEP PSHPALRQKTVAFGKTVNVS QTVEGTSRNSKKVLASTMSA QNT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	356
15N chemical shifts	107
1H chemical shifts	430

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sas-5N	1

Entities:

Entity 1, sas-5N 123 residues - Formula weight is not available

Residues 1 and 2 are cloning artifacts. Numbering in assignments corresponds to uniprot Q20010 except for the cloning artefact.

1

GLY

PRO

ASN

TYR

ASP

LEU

PRO

CYS

2

SER

ILE

TYR

PHE

LYS

PRO

THR

VAL

GLN

3

GLU

PHE

VAL

ASP

GLN

PRO

ARG

VAL

PHE

GLU

4

ASP

SER

GLU

VAL

PRO

ALA

PHE

GLN

GLU

VAL

5

LEU

GLN

LEU

PRO

GLN

GLU

ARG

THR

LYS

PRO

6

PRO

VAL

PRO

SER

THR

GLN

PRO

ILE

VAL

ALA

7

ALA

VAL

GLU

VAL

ALA

LYS

SER

CYS

8

LEU

SER

ALA

PRO

LYS

PRO

ARG

LYS

GLU

PRO

9

PRO

SER

HIS

PRO

ALA

LEU

ARG

GLN

LYS

THR

10

VAL

ALA

PHE

GLY

LYS

THR

VAL

ASN

VAL

SER

11

GLN

THR

VAL

GLU

GLY

THR

SER

ARG

ASN

SER

12

LYS

VAL

LEU

ALA

SER

THR

MET

SER

ALA

13

GLN

ASN

THR

Samples:

sample_1: sas-5N, [U-100% 13C; U-100% 15N], 0.5 mM; Potassium PIPES (K-PIPES) 80 mM; EGTA 1 mM; MgCl2 1 mM; NaN3 0.02 % w/v; DSS 50 uM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHAHB	sample_1	isotropic	sample_conditions_1

Software:

Omega_spectrometer_software vvBeta 6.0.3b2, GE - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis, CCPN - data analysis

NMR spectrometers:

Home-built OMEGA 750 MHz

UNP	Q20010
AlphaFold	G3MU16