BMRB Entry 27052

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27052

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Title:
Backbone 1H, 13C, and 15N and ALV methyl Chemical Shift Assignments of the yeast TIM9/10 complex
Deposition date:
2017-03-22
Original release date:
2017-03-31
Authors:
Weinhaeupl, Katharina; Schanda, Paul
Citation:

Citation: Weinhaeupl, Katharina; Schanda, Paul. "Backbone 1H, 13C, and 15N and ALV methyl Chemical Shift Assignments of the yeast TIM9/10 complex"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
TIM9, polymer, 87 residues, Formula weight is not available
TIM10, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TIM9: MDALNSKEQQEFQKVVEQKQ MKDFMRLYSNLVERCFTDCV NDFTTSKLTNKEQTCIMKCS EKFLKHSERVGQRFQEQNAA LGQGLGR
TIM10: GSFLGFGGGQPQLSSQQKIQ AAEAELDLVTDMFNKLVNNC YKKCINTSYSEGELNKNESS CLDRCVAKYFETNVQVGENM QKMGQSFNAAGKF

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts163
1H chemical shifts252

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TIM91
2TIM102

Entities:

Entity 1, TIM9 87 residues - Formula weight is not available

1   METASPALALEUASNSERLYSGLUGLNGLN
2   GLUPHEGLNLYSVALVALGLUGLNLYSGLN
3   METLYSASPPHEMETARGLEUTYRSERASN
4   LEUVALGLUARGCYSPHETHRASPCYSVAL
5   ASNASPPHETHRTHRSERLYSLEUTHRASN
6   LYSGLUGLNTHRCYSILEMETLYSCYSSER
7   GLULYSPHELEULYSHISSERGLUARGVAL
8   GLYGLNARGPHEGLNGLUGLNASNALAALA
9   LEUGLYGLNGLYLEUGLYARG

Entity 2, TIM10 93 residues - Formula weight is not available

1   GLYSERPHELEUGLYPHEGLYGLYGLYGLN
2   PROGLNLEUSERSERGLNGLNLYSILEGLN
3   ALAALAGLUALAGLULEUASPLEUVALTHR
4   ASPMETPHEASNLYSLEUVALASNASNCYS
5   TYRLYSLYSCYSILEASNTHRSERTYRSER
6   GLUGLYGLULEUASNLYSASNGLUSERSER
7   CYSLEUASPARGCYSVALALALYSTYRPHE
8   GLUTHRASNVALGLNVALGLYGLUASNMET
9   GLNLYSMETGLYGLNSERPHEASNALAALA
10   GLYLYSPHE

Samples:

sample_1: TIM9, [U-13C; U-15N; U-2H], 0.2 mM; TIM10 0.2 mM; MES 20 mM; NaCl 50 mM

sample_2: TIM10, [U-13C; U-15N; U-2H], 0.2 mM; TIM9 0.2 mM; MES 20 mM; NaCl 50 mM

sample_3: TIM10 0.2 mM; TIM9, [U-2H; U-15H; Ala, Leu(proS),Val(proS) methyl], 0.2 mM; MES 20 mM; NaCl 50 mM

sample_4: TIM10, [U-2H; U-15H; Ala, Leu(proS),Val(proS) methyl], 0.2 mM; TIM9 0.2 mM; MES 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D 13C-HSQC-NOESYsample_3isotropicsample_conditions_1
3D 15N-HSQC-NOESYsample_3isotropicsample_conditions_1
3D 13C-HSQC-NOESYsample_4isotropicsample_conditions_1
3D 15N-HSQC-NOESYsample_4isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks