BMRB Entry 27044

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sgt2_TPR
Published: 2017-11-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27044

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sgt2_TPR

Deposition date:

2017-03-06

Original release date:

2017-11-10

Authors:

Krysztofinska, Ewelina; Evans, Nicola; Thapaliya, Arjun; Murray, James; Morgan, Rhodri; Martinez-Lumbreras, Santiago; Isaacson, Rivka

Citation:

Citation: Krysztofinska, Ewelina; Evans, Nicola; Thapaliya, Arjun; Murray, James; Morgan, Rhodri; Martinez-Lumbreras, Santiago; Isaacson, Rivka. "Structure and Interactions of the TPR Domain of Sgt2 with Yeast Chaperones and Ybr137wp." Front Mol Biosci 4, .-. (2017).
PubMed: 29075633

Assembly members:

Assembly members:
Sgt2, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sgt2: GSMEDDAETKAKAEDLKMQG NKAMANKDYELAINKYTEAI KVLPTNAIYYANRAAAHSSL KEYDQAVKDAESAISIDPSY FRGYSRLGFAKYAQGKPEEA LEAYKKVLDIEGDNATEAMK RDYESAKKKVEQSLNLEKTV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	407
15N chemical shifts	135
1H chemical shifts	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sgt2_TPR monomer	1

Entities:

Entity 1, Sgt2_TPR monomer 140 residues - Formula weight is not available

1	GLY	SER	MET	GLU	ASP	ASP	ALA	GLU	THR	LYS
2	ALA	LYS	ALA	GLU	ASP	LEU	LYS	MET	GLN	GLY
3	ASN	LYS	ALA	MET	ALA	ASN	LYS	ASP	TYR	GLU
4	LEU	ALA	ILE	ASN	LYS	TYR	THR	GLU	ALA	ILE
5	LYS	VAL	LEU	PRO	THR	ASN	ALA	ILE	TYR	TYR
6	ALA	ASN	ARG	ALA	ALA	ALA	HIS	SER	SER	LEU
7	LYS	GLU	TYR	ASP	GLN	ALA	VAL	LYS	ASP	ALA
8	GLU	SER	ALA	ILE	SER	ILE	ASP	PRO	SER	TYR
9	PHE	ARG	GLY	TYR	SER	ARG	LEU	GLY	PHE	ALA
10	LYS	TYR	ALA	GLN	GLY	LYS	PRO	GLU	GLU	ALA
11	LEU	GLU	ALA	TYR	LYS	LYS	VAL	LEU	ASP	ILE
12	GLU	GLY	ASP	ASN	ALA	THR	GLU	ALA	MET	LYS
13	ARG	ASP	TYR	GLU	SER	ALA	LYS	LYS	LYS	VAL
14	GLU	GLN	SER	LEU	ASN	LEU	GLU	LYS	THR	VAL

Samples:

sample_1: Sgt2, [U-13C; U-15N], 0.5 mM; potassium phosphate 10 mM; sodium chloride 100 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 0.010 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, data analysis

NMR spectrometers:

Bruker DRX 500 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	GLY	SER	MET	GLU	ASP	ASP	ALA	GLU	THR	LYS
2	ALA	LYS	ALA	GLU	ASP	LEU	LYS	MET	GLN	GLY
3	ASN	LYS	ALA	MET	ALA	ASN	LYS	ASP	TYR	GLU
4	LEU	ALA	ILE	ASN	LYS	TYR	THR	GLU	ALA	ILE
5	LYS	VAL	LEU	PRO	THR	ASN	ALA	ILE	TYR	TYR
6	ALA	ASN	ARG	ALA	ALA	ALA	HIS	SER	SER	LEU
7	LYS	GLU	TYR	ASP	GLN	ALA	VAL	LYS	ASP	ALA
8	GLU	SER	ALA	ILE	SER	ILE	ASP	PRO	SER	TYR
9	PHE	ARG	GLY	TYR	SER	ARG	LEU	GLY	PHE	ALA
10	LYS	TYR	ALA	GLN	GLY	LYS	PRO	GLU	GLU	ALA
11	LEU	GLU	ALA	TYR	LYS	LYS	VAL	LEU	ASP	ILE
12	GLU	GLY	ASP	ASN	ALA	THR	GLU	ALA	MET	LYS
13	ARG	ASP	TYR	GLU	SER	ALA	LYS	LYS	LYS	VAL
14	GLU	GLN	SER	LEU	ASN	LEU	GLU	LYS	THR	VAL

1	GLY	SER	MET	GLU	ASP	ASP	ALA	GLU	THR	LYS
2	ALA	LYS	ALA	GLU	ASP	LEU	LYS	MET	GLN	GLY
3	ASN	LYS	ALA	MET	ALA	ASN	LYS	ASP	TYR	GLU
4	LEU	ALA	ILE	ASN	LYS	TYR	THR	GLU	ALA	ILE
5	LYS	VAL	LEU	PRO	THR	ASN	ALA	ILE	TYR	TYR
6	ALA	ASN	ARG	ALA	ALA	ALA	HIS	SER	SER	LEU
7	LYS	GLU	TYR	ASP	GLN	ALA	VAL	LYS	ASP	ALA
8	GLU	SER	ALA	ILE	SER	ILE	ASP	PRO	SER	TYR
9	PHE	ARG	GLY	TYR	SER	ARG	LEU	GLY	PHE	ALA
10	LYS	TYR	ALA	GLN	GLY	LYS	PRO	GLU	GLU	ALA
11	LEU	GLU	ALA	TYR	LYS	LYS	VAL	LEU	ASP	ILE
12	GLU	GLY	ASP	ASN	ALA	THR	GLU	ALA	MET	LYS
13	ARG	ASP	TYR	GLU	SER	ALA	LYS	LYS	LYS	VAL
14	GLU	GLN	SER	LEU	ASN	LEU	GLU	LYS	THR	VAL

1	GLY	SER	MET	GLU	ASP	ASP	ALA	GLU	THR	LYS
2	ALA	LYS	ALA	GLU	ASP	LEU	LYS	MET	GLN	GLY
3	ASN	LYS	ALA	MET	ALA	ASN	LYS	ASP	TYR	GLU
4	LEU	ALA	ILE	ASN	LYS	TYR	THR	GLU	ALA	ILE
5	LYS	VAL	LEU	PRO	THR	ASN	ALA	ILE	TYR	TYR
6	ALA	ASN	ARG	ALA	ALA	ALA	HIS	SER	SER	LEU
7	LYS	GLU	TYR	ASP	GLN	ALA	VAL	LYS	ASP	ALA
8	GLU	SER	ALA	ILE	SER	ILE	ASP	PRO	SER	TYR
9	PHE	ARG	GLY	TYR	SER	ARG	LEU	GLY	PHE	ALA
10	LYS	TYR	ALA	GLN	GLY	LYS	PRO	GLU	GLU	ALA
11	LEU	GLU	ALA	TYR	LYS	LYS	VAL	LEU	ASP	ILE
12	GLU	GLY	ASP	ASN	ALA	THR	GLU	ALA	MET	LYS
13	ARG	ASP	TYR	GLU	SER	ALA	LYS	LYS	LYS	VAL
14	GLU	GLN	SER	LEU	ASN	LEU	GLU	LYS	THR	VAL