BMRB Entry 27039

Name: Chemical shifts of amorphous-looking aggregates of the P23T mutant of human gamma-D-crystallin formed at pH 7.
Published: 2017-05-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27039

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of amorphous-looking aggregates of the P23T mutant of human gamma-D-crystallin formed at pH 7.

Deposition date:

2017-02-26

Original release date:

2017-05-02

Authors:

Boatz, Jennifer; Whitley, Matthew; Li, Mingyue; Gronenborn, Angela; van der Wel, Patrick

Citation:

Citation: Boatz, Jennifer; Whitley, Matthew; Li, Mingyue; Gronenborn, Angela; van der Wel, Patrick. "Cataract-associated P23T gamma-D-crystallin retains a native-like fold in amorphous-looking aggregates formed at physiological pH" Nat Commun 8, 15137-15137 (2017).
PubMed: 28474685

Assembly members:

Assembly members:
gD_crystallin_P23T, polymer, 173 residues, 20616.9178 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gD_crystallin_P23T: GKITLYEDRGFQGRHYECSS DHTNLQPYLSRCNSARVDSG CWMLYEQPNYSGLQYFLRRG DYADHQQWMGLSDSVRSCRL IPHSGSHRIRLYEREDYRGQ MIEFTEDCSCLQDRFRFNEI HSLNVLEGSWVLYELSNYRG RQYLLMPGDYRRYQDWGATN ARVGSLRRVIDFS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	157
15N chemical shifts	39

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	gDcrystallinP23T	1

Entities:

Entity 1, gDcrystallinP23T 173 residues - 20616.9178 Da.

1

GLY

LYS

ILE

THR

LEU

TYR

GLU

ASP

ARG

GLY

2

PHE

GLN

GLY

ARG

HIS

TYR

GLU

CYS

SER

3

ASP

HIS

THR

ASN

LEU

GLN

PRO

TYR

LEU

SER

4

ARG

CYS

ASN

SER

ALA

ARG

VAL

ASP

SER

GLY

5

CYS

TRP

MET

LEU

TYR

GLU

GLN

PRO

ASN

TYR

6

SER

GLY

LEU

GLN

TYR

PHE

LEU

ARG

GLY

7

ASP

TYR

ALA

ASP

HIS

GLN

TRP

MET

GLY

8

LEU

SER

ASP

SER

VAL

ARG

SER

CYS

ARG

LEU

9

ILE

PRO

HIS

SER

GLY

SER

HIS

ARG

ILE

ARG

10

LEU

TYR

GLU

ARG

GLU

ASP

TYR

ARG

GLY

GLN

11

MET

ILE

GLU

PHE

THR

GLU

ASP

CYS

SER

CYS

12

LEU

GLN

ASP

ARG

PHE

ARG

PHE

ASN

GLU

ILE

13

HIS

SER

LEU

ASN

VAL

LEU

GLU

GLY

SER

TRP

14

VAL

LEU

TYR

GLU

LEU

SER

ASN

TYR

ARG

GLY

15

ARG

GLN

TYR

LEU

MET

PRO

GLY

ASP

TYR

16

ARG

TYR

GLN

ASP

TRP

GLY

ALA

THR

ASN

17

ALA

ARG

VAL

GLY

SER

LEU

ARG

VAL

ILE

18

ASP

PHE

SER

Samples:

gDcrytallinP23T: gD crystallin P23T, [U-99% 13C; U-99% 15N], mg

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D NCACX	gDcrytallinP23T	isotropic	sample_conditions_1
3D NCOCX	gDcrytallinP23T	isotropic	sample_conditions_1
2D DARR	gDcrytallinP23T	isotropic	sample_conditions_1
2D NCACX	gDcrytallinP23T	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

UNP	P07320
AlphaFold	Q99681