BMRB Entry 27035

Name: Backbone assignment of human Ube2T
Published: 2017-04-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27035

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of human Ube2T

Deposition date:

2017-02-14

Original release date:

2017-04-25

Authors:

Bortoluzzi, Alessio; Morreale, Francesca; Walden, Helen; Ciulli, Alessio

Citation:

Citation: Morreale, Francesca; Bortoluzzi, Alessio; Chaugule, Viduth; Arkinson, Connor; Walden, Helen; Ciulli, Alessio. "Allosteric targeting of the Fanconi anemia ubiquitin-conjugating enzyme Ube2T by fragment screening" J. Med. Chem. 60, 4093-4098 (2017).
PubMed: 28437106

Assembly members:

Assembly members:
Ube2T_(1-154), polymer, 154 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ube2T_(1-154): MQRASRLKRELHMLATEPPP GITCWQDKDQMDDLRAQILG GANTPYEKGVFKLEVIIPER YPFEPPQIRFLTPIYHPNID SAGRICLDVLKLPPKGAWRP SLNIATVLTSIQLLMSEPNP DDPLMADISSEFKYNKPAFL KNARQWTEKHARQK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	399
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ube2T (1-154)	1

Entities:

Entity 1, Ube2T (1-154) 154 residues - Formula weight is not available

1

MET

GLN

ARG

ALA

SER

ARG

LEU

LYS

ARG

GLU

2

LEU

HIS

MET

LEU

ALA

THR

GLU

PRO

3

GLY

ILE

THR

CYS

TRP

GLN

ASP

LYS

ASP

GLN

4

MET

ASP

LEU

ARG

ALA

GLN

ILE

LEU

GLY

5

GLY

ALA

ASN

THR

PRO

TYR

GLU

LYS

GLY

VAL

6

PHE

LYS

LEU

GLU

VAL

ILE

PRO

GLU

ARG

7

TYR

PRO

PHE

GLU

PRO

GLN

ILE

ARG

PHE

8

LEU

THR

PRO

ILE

TYR

HIS

PRO

ASN

ILE

ASP

9

SER

ALA

GLY

ARG

ILE

CYS

LEU

ASP

VAL

LEU

10

LYS

LEU

PRO

LYS

GLY

ALA

TRP

ARG

PRO

11

SER

LEU

ASN

ILE

ALA

THR

VAL

LEU

THR

SER

12

ILE

GLN

LEU

MET

SER

GLU

PRO

ASN

PRO

13

ASP

PRO

LEU

MET

ALA

ASP

ILE

SER

14

GLU

PHE

LYS

TYR

ASN

LYS

PRO

ALA

PHE

LEU

15

LYS

ASN

ALA

ARG

GLN

TRP

THR

GLU

LYS

HIS

16

ALA

ARG

GLN

LYS

Samples:

sample_1: Ube2T (1-154), [U-100% 13C; U-100% 15N], 360 uM; potassium phosphate 50 mM; sodium chloride 85 mM; DTT 1 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection, processing

NMR spectrometers:

Bruker Avance III HD 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks