BMRB Entry 27034

Name: 1H, 13C and 15N chemical shift assignments of A. thaliana RCD1(468-569)
Published: 2017-06-13

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PDB ID: 5n9q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27034
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignments of A. thaliana RCD1(468-569)

Deposition date:

2017-02-13

Original release date:

2017-06-13

Authors:

Tossavainen, Helena; Hellman, Maarit; Vainonen, Julia; Kangasjarvi, Jaakko; Permi, Perttu

Citation:

Citation: Tossavainen, Helena; Hellman, Maarit; Vainonen, Julia; Kangasjarvi, Jaakko; Permi, Perttu. "1H, 13C and 15N NMR chemical shift assignments of A. thaliana RCD1 RST" Biomol. NMR Assignments 11, 207-210 (2017).
PubMed: 28593560

Assembly members:

Assembly members:
RCD1, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RCD1: GSPEFMGVTLEGPKDLPPQL ESNQGARGSGSANSVGSSTT RPKSPWMPFPTLFAAISHKV AENDMLLINADYQQLRDKKM TRAEFVRKLRVIVGDDLLRS TITTLQNQPKSKEIPGSIRD HEEGAGGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	500
15N chemical shifts	123
1H chemical shifts	811

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RCD1	1

Entities:

Entity 1, RCD1 128 residues - Formula weight is not available

1

GLY

SER

PRO

GLU

PHE

MET

GLY

VAL

THR

LEU

2

GLU

GLY

PRO

LYS

ASP

LEU

PRO

GLN

LEU

3

GLU

SER

ASN

GLN

GLY

ALA

ARG

GLY

SER

GLY

4

SER

ALA

ASN

SER

VAL

GLY

SER

THR

5

ARG

PRO

LYS

SER

PRO

TRP

MET

PRO

PHE

PRO

6

THR

LEU

PHE

ALA

ILE

SER

HIS

LYS

VAL

7

ALA

GLU

ASN

ASP

MET

LEU

ILE

ASN

ALA

8

ASP

TYR

GLN

LEU

ARG

ASP

LYS

MET

9

THR

ARG

ALA

GLU

PHE

VAL

ARG

LYS

LEU

ARG

10

VAL

ILE

VAL

GLY

ASP

LEU

ARG

SER

11

THR

ILE

THR

LEU

GLN

ASN

GLN

PRO

LYS

12

SER

LYS

GLU

ILE

PRO

GLY

SER

ILE

ARG

ASP

13

HIS

GLU

GLY

ALA

GLY

LEU

Samples:

sample_1: RCD1, [U-13C; U-15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D iHNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D hCCmHm	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks