BMRB Entry 27033
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27033
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Citation: Martin, Tesmine; Lou, Yuan-Chao; Aryal, Sarita; Tai, Jung-Hsiang; Chen, Chinpan. "1H, 13C and 15N resonance assignments and secondary structures of cyclophilin 2 from Trichomonas vaginalis" Biomol. NMR Assignments 12, 27-30 (2018).
PubMed: 28875299
Assembly members:
cyclophilin_2_from_Trichomonas_vaginalis, polymer, 194 residues, 20947 Da.
Natural source: Common Name: Trichomonas vaginalis Taxonomy ID: 5722 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trichomonas vaginalis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b
Entity Sequences (FASTA):
cyclophilin_2_from_Trichomonas_vaginalis: MLAFFATRVISAPKVTKKVF
FKISINGEDAGTIKFGLFGD
DVPKTAENFRALCTGEKGMG
KLGKPLHYKGSPFHRVIPNF
MIQGGDITSGNGYGGESIYG
SKFADESFKITHDGPGLLSM
ANSGPNTNGSQFFITTVPCP
WLNGKHVVFGKVIEGMEIVK
KIESLGSQSGTPKAKIIIAD
CGEITELEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 750 |
| 15N chemical shifts | 175 |
| 1H chemical shifts | 1115 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TvCyP2 monomer | 1 |
Entities:
Entity 1, TvCyP2 monomer 194 residues - 20947 Da.
| 1 | MET | LEU | ALA | PHE | PHE | ALA | THR | ARG | VAL | ILE | ||||
| 2 | SER | ALA | PRO | LYS | VAL | THR | LYS | LYS | VAL | PHE | ||||
| 3 | PHE | LYS | ILE | SER | ILE | ASN | GLY | GLU | ASP | ALA | ||||
| 4 | GLY | THR | ILE | LYS | PHE | GLY | LEU | PHE | GLY | ASP | ||||
| 5 | ASP | VAL | PRO | LYS | THR | ALA | GLU | ASN | PHE | ARG | ||||
| 6 | ALA | LEU | CYS | THR | GLY | GLU | LYS | GLY | MET | GLY | ||||
| 7 | LYS | LEU | GLY | LYS | PRO | LEU | HIS | TYR | LYS | GLY | ||||
| 8 | SER | PRO | PHE | HIS | ARG | VAL | ILE | PRO | ASN | PHE | ||||
| 9 | MET | ILE | GLN | GLY | GLY | ASP | ILE | THR | SER | GLY | ||||
| 10 | ASN | GLY | TYR | GLY | GLY | GLU | SER | ILE | TYR | GLY | ||||
| 11 | SER | LYS | PHE | ALA | ASP | GLU | SER | PHE | LYS | ILE | ||||
| 12 | THR | HIS | ASP | GLY | PRO | GLY | LEU | LEU | SER | MET | ||||
| 13 | ALA | ASN | SER | GLY | PRO | ASN | THR | ASN | GLY | SER | ||||
| 14 | GLN | PHE | PHE | ILE | THR | THR | VAL | PRO | CYS | PRO | ||||
| 15 | TRP | LEU | ASN | GLY | LYS | HIS | VAL | VAL | PHE | GLY | ||||
| 16 | LYS | VAL | ILE | GLU | GLY | MET | GLU | ILE | VAL | LYS | ||||
| 17 | LYS | ILE | GLU | SER | LEU | GLY | SER | GLN | SER | GLY | ||||
| 18 | THR | PRO | LYS | ALA | LYS | ILE | ILE | ILE | ALA | ASP | ||||
| 19 | CYS | GLY | GLU | ILE | THR | GLU | LEU | GLU | HIS | HIS | ||||
| 20 | HIS | HIS | HIS | HIS |
Samples:
sample_1: cyclophilin 2 from Trichomonas vaginalis, [U-100% 13C; U-100% 15N], 0.6 ± 0.01 mM
sample_conditions_1: ionic strength: 170 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.1, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks