BMRB Entry 27026

Name: Ascl1 fragment D
Published: 2017-04-21

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27026

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Ascl1 fragment D

Deposition date:

2017-02-06

Original release date:

2017-04-21

Authors:

Baronti, Lorenzo; Hosek, Tomas; Gil-Caballero, Sergio; Raveh-Amit, Hadas; Calcada, Eduardo; Ayala, Isabel; Dinnyes, Andras; Felli, Isabella; Pierattelli, Roberta; Brutscher, Bernhard

Citation:

Citation: Baronti, Lorenzo; Hosek, Tomas; Gil-Caballero, Sergio; Raveh-Amit, Hadas; Calcada, Eduardo; Ayala, Isabel; Dinnyes, Andras; Felli, Isabella; Pierattelli, Roberta; Brutscher, Bernhard. "Fragment-Based NMR Study of the Conformational Dynamics in the bHLH Transcription Factor Ascl1'" Biophys. J. 112, 1366-1373 (2017).
PubMed: 28402879

Assembly members:

Assembly members:
Ascl1_fragment_D, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ascl1_fragment_D: MSYYHHHHHHLESTSLYKKA GLYKKAGSAAAVLEENLYFQ GSFTAAVARRNERERNRVKL VNLGFATLREHVPNGAANKK MSKVETLRSAVEYIRALQQL LDEHDAVSAAFQAGVLSPTI SPNYSNDLNSMAGSPVSSYS SDEGSYDPLSPEEQELLDFT NWF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	237
15N chemical shifts	83
1H chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ascl1 trascription factor, Fragment D	1

Entities:

Entity 1, Ascl1 trascription factor, Fragment D 163 residues - Formula weight is not available

Residues 1-44 represent the artificial affinity and solubility tag. Ascl1 fragment D chemical shift assignment start from residue 45 (residue 1 in the assignment file).

1

MET

SER

TYR

HIS

2

LEU

GLU

SER

THR

SER

LEU

TYR

LYS

ALA

3

GLY

LEU

TYR

LYS

ALA

GLY

SER

ALA

4

ALA

VAL

LEU

GLU

ASN

LEU

TYR

PHE

GLN

5

GLY

SER

PHE

THR

ALA

VAL

ALA

ARG

6

ASN

GLU

ARG

GLU

ARG

ASN

ARG

VAL

LYS

LEU

7

VAL

ASN

LEU

GLY

PHE

ALA

THR

LEU

ARG

GLU

8

HIS

VAL

PRO

ASN

GLY

ALA

ASN

LYS

9

MET

SER

LYS

VAL

GLU

THR

LEU

ARG

SER

ALA

10

VAL

GLU

TYR

ILE

ARG

ALA

LEU

GLN

LEU

11

LEU

ASP

GLU

HIS

ASP

ALA

VAL

SER

ALA

12

PHE

GLN

ALA

GLY

VAL

LEU

SER

PRO

THR

ILE

13

SER

PRO

ASN

TYR

SER

ASN

ASP

LEU

ASN

SER

14

MET

ALA

GLY

SER

PRO

VAL

SER

TYR

SER

15

SER

ASP

GLU

GLY

SER

TYR

ASP

PRO

LEU

SER

16

PRO

GLU

GLN

GLU

LEU

ASP

PHE

THR

17

ASN

TRP

PHE

Samples:

sample_1: Ascl1 fragment D, [U-100% 13C; U-100% 15N], 250 uM; TRIS 50 mM; DTT 5 mM; SDS 2 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D BEST-TROSY HCNs	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HNNs	sample_1	isotropic	sample_conditions_1
HADAMAC	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 950 MHz
Bruker Avance 850 MHz
Bruker Avance 700 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks