BMRB Entry 27025

Name: Ascl1 fragment C
Published: 2017-04-21

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27025

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Ascl1 fragment C

Deposition date:

2017-02-06

Original release date:

2017-04-21

Authors:

Baronti, Lorenzo; Hosek, Tomas; Gil-Caballero, Sergio; Raveh-Amit, Hadas; Calcada, Eduardo; Ayala, Isabel; Dinnyes, Andras; Felli, Isabella; Pierattelli, Roberta; Brutscher, Bernhard

Citation:

Citation: Baronti, Lorenzo; Hosek, Tomas; Gil-Caballero, Sergio; Raveh-Amit, Hadas; Calcada, Eduardo; Ayala, Isabel; Dinnyes, Andras; Felli, Isabella; Pierattelli, Roberta; Brutscher, Bernhard. "Fragment-Based NMR Study of the Conformational Dynamics in the bHLH Transcription Factor Ascl1'" Biophys. J. 112, 1366-1373 (2017).
PubMed: 28402879

Assembly members:

Assembly members:
Ascl1_fragment_C, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ascl1_fragment_C: MASHHHHHHQQQPAAVARRN ERERNRVKLVNLGFATLREH VPNGAANKKMSKVETLRSAV EYIRALQQLLDEHDAVSAA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	208
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ascl1 trascription factor, Fragment C	1

Entities:

Entity 1, Ascl1 trascription factor, Fragment C 79 residues - Formula weight is not available

Residues 1-9 represent the artificial affinity tag. Ascl1 fragment C chemical shift assignment start from residue 10 (residue 1 in the assignment file).

1

MET

ALA

SER

HIS

GLN

2

GLN

PRO

ALA

VAL

ALA

ARG

ASN

3

GLU

ARG

GLU

ARG

ASN

ARG

VAL

LYS

LEU

VAL

4

ASN

LEU

GLY

PHE

ALA

THR

LEU

ARG

GLU

HIS

5

VAL

PRO

ASN

GLY

ALA

ASN

LYS

MET

6

SER

LYS

VAL

GLU

THR

LEU

ARG

SER

ALA

VAL

7

GLU

TYR

ILE

ARG

ALA

LEU

GLN

LEU

8

ASP

GLU

HIS

ASP

ALA

VAL

SER

ALA

Samples:

sample_1: Ascl1 fragment C, [U-100% 13C; U-100% 15N], 250 uM; TRIS 50 mM; DTT 5 mM; SDS 2 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 323 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D BEST-TROSY HCNs	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HNNs	sample_1	isotropic	sample_conditions_1
HADAMAC	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 950 MHz
Bruker Avance 850 MHz
Bruker Avance 700 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks