BMRB Entry 27020

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human BetaB2-crystallin
Published: 2017-03-31

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27020

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human BetaB2-crystallin

Deposition date:

2017-02-01

Original release date:

2017-03-31

Authors:

Xi, Zhaoyong; Whitley, Matthew; Gronenborn, Angela

Citation:

Citation: Xi, Zhaoyong; Whitley, Matthew; Gronenborn, Angela. "Human betaB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study" Structure 25, 496-505 (2017).
PubMed: 28238532

Assembly members:

Assembly members:
human_betaB2-crystallin, polymer, 204 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_betaB2-crystallin: ASDHQTQAGKPQSLNPKIII FEQENFQGHSHELNGPCPNL KETGVEKAGSVLVQAGPWVG YEQANCKGEQFVFEKGEYPR WDSWTSSRRTDSLSSLRPIK VDSQEHKIILYENPNFTGKK MEIIDDDVPSFHAHGYQEKV SSVRVQSGTWVGYQYPGYRG LQYLLEKGDYKDSSDFGAPH PQVQSVRRIRDMQWHQRGAF HPSN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	322
15N chemical shifts	162
1H chemical shifts	162

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human betaB2-crystallin, chain 1	1
2	human betaB2-crystallin, chain 2	1

Entities:

Entity 1, human betaB2-crystallin, chain 1 204 residues - Formula weight is not available

1

ALA

SER

ASP

HIS

GLN

THR

GLN

ALA

GLY

LYS

2

PRO

GLN

SER

LEU

ASN

PRO

LYS

ILE

3

PHE

GLU

GLN

GLU

ASN

PHE

GLN

GLY

HIS

SER

4

HIS

GLU

LEU

ASN

GLY

PRO

CYS

PRO

ASN

LEU

5

LYS

GLU

THR

GLY

VAL

GLU

LYS

ALA

GLY

SER

6

VAL

LEU

VAL

GLN

ALA

GLY

PRO

TRP

VAL

GLY

7

TYR

GLU

GLN

ALA

ASN

CYS

LYS

GLY

GLU

GLN

8

PHE

VAL

PHE

GLU

LYS

GLY

GLU

TYR

PRO

ARG

9

TRP

ASP

SER

TRP

THR

SER

ARG

THR

10

ASP

SER

LEU

SER

LEU

ARG

PRO

ILE

LYS

11

VAL

ASP

SER

GLN

GLU

HIS

LYS

ILE

LEU

12

TYR

GLU

ASN

PRO

ASN

PHE

THR

GLY

LYS

13

MET

GLU

ILE

ASP

VAL

PRO

SER

14

PHE

HIS

ALA

HIS

GLY

TYR

GLN

GLU

LYS

VAL

15

SER

VAL

ARG

VAL

GLN

SER

GLY

THR

TRP

16

VAL

GLY

TYR

GLN

TYR

PRO

GLY

TYR

ARG

GLY

17

LEU

GLN

TYR

LEU

GLU

LYS

GLY

ASP

TYR

18

LYS

ASP

SER

ASP

PHE

GLY

ALA

PRO

HIS

19

PRO

GLN

VAL

GLN

SER

VAL

ARG

ILE

ARG

20

ASP

MET

GLN

TRP

HIS

GLN

ARG

GLY

ALA

PHE

21

HIS

PRO

SER

ASN

Samples:

sample_1: human betaB2-crystallin, [U-13C; U-15N; U-2H], 0.625 mM; sodium phosphate buffer 25 mM; EDTA 1 mM; DTT 5 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks