BMRB Entry 27019
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27019
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Citation: Bruell, Shoni; Sethi, Ashish; Smith, Nicholas; Scott, Daniel; Hossain, Mohammed Akhter; Wu, Qing-Ping; Guo, Zhan-Yun; Petrie, Emma; Gooley, Paul; Bathgate, Ross. "The involvement of the LDLa linker in the mode of activation of the GPCR RXFP2" .
Assembly members:
RXFP2-LDLa-linker-GB1, polymer, 121 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15
Entity Sequences (FASTA):
RXFP2-LDLa-linker-GB1: MITFSCQKGYFPCGNLTKCL
PRAFHCDGKDDCGNGADEEN
CGDTSGWATIFGTVHGNANS
VALTQEQYKLALNGKTLKGE
TTTEAVDAATAEKVFKQYAN
DNGVDGEWTYDDATKTFTVT
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 424 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 566 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RXFP2-LDLa-linker-GB1 | 1 |
| 2 | Calcium | 2 |
Entities:
Entity 1, RXFP2-LDLa-linker-GB1 121 residues - Formula weight is not available
| 1 | MET | ILE | THR | PHE | SER | CYS | GLN | LYS | GLY | TYR | ||||
| 2 | PHE | PRO | CYS | GLY | ASN | LEU | THR | LYS | CYS | LEU | ||||
| 3 | PRO | ARG | ALA | PHE | HIS | CYS | ASP | GLY | LYS | ASP | ||||
| 4 | ASP | CYS | GLY | ASN | GLY | ALA | ASP | GLU | GLU | ASN | ||||
| 5 | CYS | GLY | ASP | THR | SER | GLY | TRP | ALA | THR | ILE | ||||
| 6 | PHE | GLY | THR | VAL | HIS | GLY | ASN | ALA | ASN | SER | ||||
| 7 | VAL | ALA | LEU | THR | GLN | GLU | GLN | TYR | LYS | LEU | ||||
| 8 | ALA | LEU | ASN | GLY | LYS | THR | LEU | LYS | GLY | GLU | ||||
| 9 | THR | THR | THR | GLU | ALA | VAL | ASP | ALA | ALA | THR | ||||
| 10 | ALA | GLU | LYS | VAL | PHE | LYS | GLN | TYR | ALA | ASN | ||||
| 11 | ASP | ASN | GLY | VAL | ASP | GLY | GLU | TRP | THR | TYR | ||||
| 12 | ASP | ASP | ALA | THR | LYS | THR | PHE | THR | VAL | THR | ||||
| 13 | GLU |
Entity 2, Calcium - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: RXFP2-LDLa-linker-GB1, [U-100% 15N], 300 uM; RXFP2-LDLa-linker-GB1, [U-100% 13C; U-100% 15N], 350 uM
sample_conditions_1: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks