BMRB Entry 27011

Name: Relaxation data for sigma1.1 from Bacillus subtilis
Published: 2017-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27011

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Relaxation data for sigma1.1 from Bacillus subtilis

Deposition date:

2017-01-20

Original release date:

2017-06-30

Authors:

Zachrdla, Milan; Padrta, Petr; Rabatinova, Alzbeta; Sanderova, Hana; Barvik, Ivan; Krasny, Libor; Zidek, Lukas

Citation:

Citation: Zachrdla, Milan; Padrta, Petr; Rabatinova, Alzbeta; Sanderova, Hana; Barvik, Ivan; Krasny, Libor; Zidek, Lukas. "Solution Structure of Domain 1.1 of the sigma(A) Factor from Bacillus subtilis is Preformed for Binding to the RNA Polymerase Core" J. Biol. Chem. 292, 11610-11617 (2017).
PubMed: 28539362

Assembly members:

Assembly members:
sigma1.1, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sigma1.1: ADKQTHETELTFDQVKEQLT ESGKKRGVLTYEEIAERMSS FEIESDQMDEYYEFLGEQGV ELISENEETEDLEHHHHHH

Data sets:

heteronucl_NOEs
heteronucl_T1_relaxation
heteronucl_T2_relaxation
order_parameters
- order_parameter_list_1

Data type	Count
T1 relaxation values	204
T2 relaxation values	206
heteronuclear NOE values	204
order parameters	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	domain1.1	1

Entities:

Entity 1, domain1.1 79 residues - Formula weight is not available

1

ALA

ASP

LYS

GLN

THR

HIS

GLU

THR

GLU

LEU

2

THR

PHE

ASP

GLN

VAL

LYS

GLU

GLN

LEU

THR

3

GLU

SER

GLY

LYS

ARG

GLY

VAL

LEU

THR

4

TYR

GLU

ILE

ALA

GLU

ARG

MET

SER

5

PHE

GLU

ILE

GLU

SER

ASP

GLN

MET

ASP

GLU

6

TYR

GLU

PHE

LEU

GLY

GLU

GLN

GLY

VAL

7

GLU

LEU

ILE

SER

GLU

ASN

GLU

THR

GLU

8

ASP

LEU

GLU

HIS

Samples:

sample_1: sigma1.1, [U-100% 15N], 0.6 mM; sodium chloride 10 mM; sodium azide 6 mM; phosphate buffer 20 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.6; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D R1 15N,1H correlation	sample_1	isotropic	sample_conditions_1
2D R2 15N,1H correlation	sample_1	isotropic	sample_conditions_1
2D NOE 15N,1H correlation	sample_1	isotropic	sample_conditions_1
2D 15N1H correlation - transverse cross-correlated cross-relaxation (symmetrical reconversion)	sample_1	isotropic	sample_conditions_1
2D 15N1H correlation - longitudinal cross-correlated cross-relaxation (symmetrical reconversion)	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

Relax, d'Auvergne - data analysis

Octave, Eaton - data analysis

NMR spectrometers:

Bruker Avance 950 MHz
Bruker Avance 850 MHz
Bruker Avance 600 MHz