BMRB Entry 27002

Name: Assignment of HuR RRM1-RRM2 tandem domain
Published: 2017-11-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27002

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of HuR RRM1-RRM2 tandem domain

Deposition date:

2017-01-19

Original release date:

2017-11-16

Authors:

Lal, Preet; Cerofolini, Linda; D'Agostino, Vito Giuseppe; Zucal, Chiara; Fuccio, Carmelo; Bonomo, Isabelle; Luchinat, Claudio; Di Maio, Danilo; Novellino, Ettore; Biasini, Emiliano; Elezgarai, Saioa; Manzoni, Leonardo; Seneci, Pierfausto; Marinelli, Luciana; Fragai, Marco; Provenzani, Alessandro

Citation:

Citation: Lal, Preet; Cerofolini, Linda; D'Agostino, Vito Giuseppe; Zucal, Chiara; Fuccio, Carmelo; Bonomo, Isabelle; Dassi, Erik; Giuntini, Stefano; Di Maio, Danilo; Vishwakarma, Vikalp; Preet, Ranjan; Williams, Sha Neisha; Fairlamb, Max; Munk, Rachel; Lehrmann, Elin; Abdelmohsen, Kotb; Elezgarai, Saioa; Luchinat, Claudio; Novellino, Ettore; Quattrone, Alessandro; Biasini, Emiliano; Manzoni, Leonardo; Gorospe, Myriam; Dixon, Dan; Seneci, Pierfausto; Marinelli, Luciana; Fragai, Marco; Provenzani, Alessandro. "Regulation of HuR structure and function by dihydrotanshinone-I." Nucleic Acids Res. 45, 9514-9527 (2017).
PubMed: 28934484

Assembly members:

Assembly members:
HuR_RRM1-RRM2, polymer, 210 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-42a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HuR_RRM1-RRM2: MSNGYEDHMAEDCRGDIGRT NLIVNYLPQNMTQDELRSLF SSIGEVESAKLIRDKVAGHS LGYGFVNYVTAKDAERAINT LNGLRLQSKTIKVSYARPSS EVIKDANLYISGLPRTMTQK DVEDMFSRFGRIINSRVLVD QTTGLSRGVAFIRFDKRSEA EEAITSFNGHKPPGSSEPIA VKFAANPNQNKNVALLSPRA PPPPPPPLID

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	581
15N chemical shifts	193
1H chemical shifts	195

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HuR RRM-RRM2	1

Entities:

Entity 1, HuR RRM-RRM2 210 residues - Formula weight is not available

1	MET	SER	ASN	GLY	TYR	GLU	ASP	HIS	MET	ALA
2	GLU	ASP	CYS	ARG	GLY	ASP	ILE	GLY	ARG	THR
3	ASN	LEU	ILE	VAL	ASN	TYR	LEU	PRO	GLN	ASN
4	MET	THR	GLN	ASP	GLU	LEU	ARG	SER	LEU	PHE
5	SER	SER	ILE	GLY	GLU	VAL	GLU	SER	ALA	LYS
6	LEU	ILE	ARG	ASP	LYS	VAL	ALA	GLY	HIS	SER
7	LEU	GLY	TYR	GLY	PHE	VAL	ASN	TYR	VAL	THR
8	ALA	LYS	ASP	ALA	GLU	ARG	ALA	ILE	ASN	THR
9	LEU	ASN	GLY	LEU	ARG	LEU	GLN	SER	LYS	THR
10	ILE	LYS	VAL	SER	TYR	ALA	ARG	PRO	SER	SER
11	GLU	VAL	ILE	LYS	ASP	ALA	ASN	LEU	TYR	ILE
12	SER	GLY	LEU	PRO	ARG	THR	MET	THR	GLN	LYS
13	ASP	VAL	GLU	ASP	MET	PHE	SER	ARG	PHE	GLY
14	ARG	ILE	ILE	ASN	SER	ARG	VAL	LEU	VAL	ASP
15	GLN	THR	THR	GLY	LEU	SER	ARG	GLY	VAL	ALA
16	PHE	ILE	ARG	PHE	ASP	LYS	ARG	SER	GLU	ALA
17	GLU	GLU	ALA	ILE	THR	SER	PHE	ASN	GLY	HIS
18	LYS	PRO	PRO	GLY	SER	SER	GLU	PRO	ILE	ALA
19	VAL	LYS	PHE	ALA	ALA	ASN	PRO	ASN	GLN	ASN
20	LYS	ASN	VAL	ALA	LEU	LEU	SER	PRO	ARG	ALA
21	PRO	PRO	PRO	PRO	PRO	PRO	PRO	LEU	ILE	ASP

Samples:

sample_1: HuR RRM1-RRM2, [U-100% 13C; U-100% 15N], 0.4 mM; MES 20 mM; sodium chloride 100 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D CON	sample_1	isotropic	sample_conditions_1
2D CACO	sample_1	isotropic	sample_conditions_1
2D CBCACO	sample_1	isotropic	sample_conditions_1
3D CBCANCO	sample_1	isotropic	sample_conditions_1
3D CBCACON	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

TOPSPIN v2.1, Bruker Biospin - processing

NMR spectrometers:

Bruker Avance 950 MHz
Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	SER	ASN	GLY	TYR	GLU	ASP	HIS	MET	ALA
2	GLU	ASP	CYS	ARG	GLY	ASP	ILE	GLY	ARG	THR
3	ASN	LEU	ILE	VAL	ASN	TYR	LEU	PRO	GLN	ASN
4	MET	THR	GLN	ASP	GLU	LEU	ARG	SER	LEU	PHE
5	SER	SER	ILE	GLY	GLU	VAL	GLU	SER	ALA	LYS
6	LEU	ILE	ARG	ASP	LYS	VAL	ALA	GLY	HIS	SER
7	LEU	GLY	TYR	GLY	PHE	VAL	ASN	TYR	VAL	THR
8	ALA	LYS	ASP	ALA	GLU	ARG	ALA	ILE	ASN	THR
9	LEU	ASN	GLY	LEU	ARG	LEU	GLN	SER	LYS	THR
10	ILE	LYS	VAL	SER	TYR	ALA	ARG	PRO	SER	SER
11	GLU	VAL	ILE	LYS	ASP	ALA	ASN	LEU	TYR	ILE
12	SER	GLY	LEU	PRO	ARG	THR	MET	THR	GLN	LYS
13	ASP	VAL	GLU	ASP	MET	PHE	SER	ARG	PHE	GLY
14	ARG	ILE	ILE	ASN	SER	ARG	VAL	LEU	VAL	ASP
15	GLN	THR	THR	GLY	LEU	SER	ARG	GLY	VAL	ALA
16	PHE	ILE	ARG	PHE	ASP	LYS	ARG	SER	GLU	ALA
17	GLU	GLU	ALA	ILE	THR	SER	PHE	ASN	GLY	HIS
18	LYS	PRO	PRO	GLY	SER	SER	GLU	PRO	ILE	ALA
19	VAL	LYS	PHE	ALA	ALA	ASN	PRO	ASN	GLN	ASN
20	LYS	ASN	VAL	ALA	LEU	LEU	SER	PRO	ARG	ALA
21	PRO	PRO	PRO	PRO	PRO	PRO	PRO	LEU	ILE	ASP

1	MET	SER	ASN	GLY	TYR	GLU	ASP	HIS	MET	ALA
2	GLU	ASP	CYS	ARG	GLY	ASP	ILE	GLY	ARG	THR
3	ASN	LEU	ILE	VAL	ASN	TYR	LEU	PRO	GLN	ASN
4	MET	THR	GLN	ASP	GLU	LEU	ARG	SER	LEU	PHE
5	SER	SER	ILE	GLY	GLU	VAL	GLU	SER	ALA	LYS
6	LEU	ILE	ARG	ASP	LYS	VAL	ALA	GLY	HIS	SER
7	LEU	GLY	TYR	GLY	PHE	VAL	ASN	TYR	VAL	THR
8	ALA	LYS	ASP	ALA	GLU	ARG	ALA	ILE	ASN	THR
9	LEU	ASN	GLY	LEU	ARG	LEU	GLN	SER	LYS	THR
10	ILE	LYS	VAL	SER	TYR	ALA	ARG	PRO	SER	SER
11	GLU	VAL	ILE	LYS	ASP	ALA	ASN	LEU	TYR	ILE
12	SER	GLY	LEU	PRO	ARG	THR	MET	THR	GLN	LYS
13	ASP	VAL	GLU	ASP	MET	PHE	SER	ARG	PHE	GLY
14	ARG	ILE	ILE	ASN	SER	ARG	VAL	LEU	VAL	ASP
15	GLN	THR	THR	GLY	LEU	SER	ARG	GLY	VAL	ALA
16	PHE	ILE	ARG	PHE	ASP	LYS	ARG	SER	GLU	ALA
17	GLU	GLU	ALA	ILE	THR	SER	PHE	ASN	GLY	HIS
18	LYS	PRO	PRO	GLY	SER	SER	GLU	PRO	ILE	ALA
19	VAL	LYS	PHE	ALA	ALA	ASN	PRO	ASN	GLN	ASN
20	LYS	ASN	VAL	ALA	LEU	LEU	SER	PRO	ARG	ALA
21	PRO	PRO	PRO	PRO	PRO	PRO	PRO	LEU	ILE	ASP

1	MET	SER	ASN	GLY	TYR	GLU	ASP	HIS	MET	ALA
2	GLU	ASP	CYS	ARG	GLY	ASP	ILE	GLY	ARG	THR
3	ASN	LEU	ILE	VAL	ASN	TYR	LEU	PRO	GLN	ASN
4	MET	THR	GLN	ASP	GLU	LEU	ARG	SER	LEU	PHE
5	SER	SER	ILE	GLY	GLU	VAL	GLU	SER	ALA	LYS
6	LEU	ILE	ARG	ASP	LYS	VAL	ALA	GLY	HIS	SER
7	LEU	GLY	TYR	GLY	PHE	VAL	ASN	TYR	VAL	THR
8	ALA	LYS	ASP	ALA	GLU	ARG	ALA	ILE	ASN	THR
9	LEU	ASN	GLY	LEU	ARG	LEU	GLN	SER	LYS	THR
10	ILE	LYS	VAL	SER	TYR	ALA	ARG	PRO	SER	SER
11	GLU	VAL	ILE	LYS	ASP	ALA	ASN	LEU	TYR	ILE
12	SER	GLY	LEU	PRO	ARG	THR	MET	THR	GLN	LYS
13	ASP	VAL	GLU	ASP	MET	PHE	SER	ARG	PHE	GLY
14	ARG	ILE	ILE	ASN	SER	ARG	VAL	LEU	VAL	ASP
15	GLN	THR	THR	GLY	LEU	SER	ARG	GLY	VAL	ALA
16	PHE	ILE	ARG	PHE	ASP	LYS	ARG	SER	GLU	ALA
17	GLU	GLU	ALA	ILE	THR	SER	PHE	ASN	GLY	HIS
18	LYS	PRO	PRO	GLY	SER	SER	GLU	PRO	ILE	ALA
19	VAL	LYS	PHE	ALA	ALA	ASN	PRO	ASN	GLN	ASN
20	LYS	ASN	VAL	ALA	LEU	LEU	SER	PRO	ARG	ALA
21	PRO	PRO	PRO	PRO	PRO	PRO	PRO	LEU	ILE	ASP