BMRB Entry 26993

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26993

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Title:
Isolated voltage-sensing domain from second subunit of human sodium channel Nav1.4 in the LPPG micelles
Deposition date:
2017-01-09
Original release date:
2017-01-19
Authors:
Myshkin, Mikhail; Paramonov, Alexander; Arseniev, Alexander; Shenkarev, Zakhar
Citation:

Citation: Paramonov, Alexander; Lyukmanova, Ekaterina; Myshkin, Mikhail; Shulepko, Mikhail; Kulbatskii, Dmitrii; Petrosian, Nikolay; Chugunov, Anton; Dolgikh, Dmitrii; Kirpichnikov, Mikhail; Arseniev, Alexander; Shenkarev, Zakhar. "NMR investigation of the isolated second voltage-sensing domain of human Nav1.4 channel"  Biochim. Biophys. Acta 1859, 493-506 (2017).
PubMed: 28065835

Assembly members:

Assembly members:
Nav1.4-II, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: pIVEX2.3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nav1.4-II: MGKVLIWNSSAPWLKFKNII HLIVMDPFVDLGITISIVLN TLFMAMEHYPMTEHFDNVLT VGNLVFTGIFTAEMVLKLIA MDPYEYFQQGWNIFDSIIVT LSLVELGLANVQGLSVLRSF RLLRVFKLAKSWPTLNMLIK IIGNSGSHHHHHH

Data sets:
Data typeCount
13C chemical shifts489
15N chemical shifts146
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NavII1

Entities:

Entity 1, NavII 153 residues - Formula weight is not available

6-His tag at the C-terminus

1   METGLYLYSVALLEUILETRPASNSERSER
2   ALAPROTRPLEULYSPHELYSASNILEILE
3   HISLEUILEVALMETASPPROPHEVALASP
4   LEUGLYILETHRILESERILEVALLEUASN
5   THRLEUPHEMETALAMETGLUHISTYRPRO
6   METTHRGLUHISPHEASPASNVALLEUTHR
7   VALGLYASNLEUVALPHETHRGLYILEPHE
8   THRALAGLUMETVALLEULYSLEUILEALA
9   METASPPROTYRGLUTYRPHEGLNGLNGLY
10   TRPASNILEPHEASPSERILEILEVALTHR
11   LEUSERLEUVALGLULEUGLYLEUALAASN
12   VALGLNGLYLEUSERVALLEUARGSERPHE
13   ARGLEULEUARGVALPHELYSLEUALALYS
14   SERTRPPROTHRLEUASNMETLEUILELYS
15   ILEILEGLYASNSERGLYSERHISHISHIS
16   HISHISHIS

Samples:

sample_1: Nav1.4-II, [U-99% 13C; U-99% 15N], 0.6 mM; LPPG 5%

Sel1_CN-Ile: Nav1.4-II, [U-13C; U-15N]-Ile, 0.1 mM; LPPG 5%

Sel2_Leu_Val: Nav1.4-II, [U-15N]-Leu; [13C(1)]-Val, 0.1 mM; LPPG 5%

Sel3_TIFW: Nav1.4-II, [U-15N]-Thr; [13C(1)]-Ile; [13C(1)]-Phe; [U-13C; U-15N]-Trp], 0.1 mM; LPPG 5%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.0; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCSel1_CN-Ileisotropicsample_conditions_1
2D HNCO 1H-15NSel1_CN-Ileisotropicsample_conditions_1
2D HNCA 1H-15NSel1_CN-Ileisotropicsample_conditions_1
3D 1H-15N NOESYSel1_CN-Ileisotropicsample_conditions_1
2D 1H-15N HSQCSel2_Leu_Valisotropicsample_conditions_1
2D HNCO 1H-15NSel2_Leu_Valisotropicsample_conditions_1
2D HNCA 1H-15NSel2_Leu_Valisotropicsample_conditions_1
2D 1H-15N HSQCSel3_TIFWisotropicsample_conditions_1
2D HNCO 1H-15NSel3_TIFWisotropicsample_conditions_1
2D HNCA 1H-15NSel3_TIFWisotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

MDDNMR, Vladislav Orekhov, Maxim Mayzel - processing

CARA, Rochus Keller - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P35499
AlphaFold Q7Z6B1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks