BMRB Entry 26986

Name: 1H, 13C and 15N Chemical shift assignments of yeast thioredoxin Saccharomyces cerevisiae in the oxidized state by solution NMR spectroscopy.
Published: 2017-08-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26986

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N Chemical shift assignments of yeast thioredoxin Saccharomyces cerevisiae in the oxidized state by solution NMR spectroscopy.

Deposition date:

2016-12-29

Original release date:

2017-08-18

Authors:

Iqbal, Anwar; Valente, Ana Paula; Almeida, Fabio

Citation:

Citation: Iqbal, Anwar; Almeida, Fabio. "1H, 13C and 15N chemical shift assignments of Saccharomyces cerevisiae type 1 thioredoxin in the oxidized state by solution NMR spectroscopy" Biomol. NMR Assign. 11, 221-224 (2017).
PubMed: 28808882

Assembly members:

Assembly members:
oxidized_yeast_thioredoxin_1, polymer, 103 residues, 11234.98 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
oxidized_yeast_thioredoxin_1: MVTQFKTASEFDSAIAQDKL VVVDFYATWCGPCKMIAPMI EKFSEQYPQADFYKLDVDEL GDVAQKNEVSAMPTLLLFKN GKEVAKVVGANPAAIKQAIA ANA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	446
15N chemical shifts	104
1H chemical shifts	718

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yTrx1	1

Entities:

Entity 1, yTrx1 103 residues - 11234.98 Da.

Full length

1

MET

VAL

THR

GLN

PHE

LYS

THR

ALA

SER

GLU

2

PHE

ASP

SER

ALA

ILE

ALA

GLN

ASP

LYS

LEU

3

VAL

ASP

PHE

TYR

ALA

THR

TRP

CYS

4

GLY

PRO

CYS

LYS

MET

ILE

ALA

PRO

MET

ILE

5

GLU

LYS

PHE

SER

GLU

GLN

TYR

PRO

GLN

ALA

6

ASP

PHE

TYR

LYS

LEU

ASP

VAL

ASP

GLU

LEU

7

GLY

ASP

VAL

ALA

GLN

LYS

ASN

GLU

VAL

SER

8

ALA

MET

PRO

THR

LEU

PHE

LYS

ASN

9

GLY

LYS

GLU

VAL

ALA

LYS

VAL

GLY

ALA

10

ASN

PRO

ALA

ILE

LYS

GLN

ALA

ILE

ALA

11

ALA

ASN

ALA

Samples:

sample_1: oxidized yeast thioredoxin 1, [U-100% 13C; U-100% 15N], 0.2 ± 0.01 mM; Sodium Phophate Buffer 20 mM

sample_conditions_1: ionic strength: 0.041 M; pH: 7.0; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance III 600 MHz
Bruker Avance III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks