BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26984

Title: Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein   PubMed: 28739907

Deposition date: 2016-12-21 Original release date: 2017-08-18

Authors: Sekar, Giridhar; ., Cowburn

Citation: Stevens, Adam; ., Sekar; H., .; ., .; ., .; 26984, .. "A promiscuous split intein with expanded protein engineering applications"  Proc. Natl. Acad. Sci. U. S. A. 114, 8538-8543 (2017).

Assembly members:
Npu_DnaE, polymer, 144 residues, 16403.5412 Da.

Natural source:   Common Name: 26984   Taxonomy ID: 272131   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
Npu_DnaE: AEYALSYETEILTVEYGLLP IGKIVEKRIECTVYSVDNNG NIYTQPVAQWHDRGEQEVFE YCLEDGSLIRATKDHKFMTV DGQMLPIDEIFERELDLMRV DNLPNMIKIATRKYLGKQNV YDIGVGEPHNFALKNGFIAS ACGN

Data sets:
Data typeCount
13C chemical shifts412
1411H chemical shifts

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Fused Npu DnaE GEP loop mutant1

Entities:

Entity 1, Fused Npu DnaE GEP loop mutant 144 residues - 16403.5412 Da.

1   ALA.269845.TYR.2698411.
2   THR.2698417.LEU.2698423.
3   VAL.2698429.CYS.2698435.
4   ASP.2698441.TYR.2698447.
5   GLN.2698453.GLU.2698459.
6   TYR.2698465.SER.2698471.
7   LYS.2698477.THR.2698483.
8   LEU.2698489.PHE.2698495.
9   LEU.26984101.LEU.26984107.
10   ILE.26984113.LEU.26984119.
11   TYR.26984125.GLU.26984131.
12   LEU.26984137.ALA.26984143.

Samples:

sample_1: sodium phosphate' 'natural abundance; .; 1, $Npu_DnaE,

CondSet1: pH: 6.0; 1: . 26984; .: K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicCondSet1
nosotropicnot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$CondSet1not available

Software:

CcpNmr_Analysis v2.4, CCPN - Spectrum processing, 26984

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts