BMRB Entry 26981

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26981

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Title:
Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein
Deposition date:
2016-12-21
Original release date:
2017-08-18
Authors:
Sekar, Giridhar; Cowburn, David
Citation:

Citation: Stevens, Adam; Sekar, Giridhar; Shah, Neel; Mostafavi, Ana; Cowburn, David; Muir, Tom. "A promiscuous split intein with expanded protein engineering applications"  Proc. Natl. Acad. Sci. U. S. A. 114, 8538-8543 (2017).
PubMed: 28739907

Assembly members:

Assembly members:
Npu_DnaE, polymer, 144 residues, 16493.6656 Da.

Natural source:

Natural source:   Common Name: Nostoc punctiforme   Taxonomy ID: 272131   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc punctiforme

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Npu_DnaE: AEYALSYETEILTVEYGLLP IGKIVEKRIECTVYSVDNNG NIYTQPVAQWHDRGEQEVFE YCLEDGSLIRATKDHKFMTV DGQMLPIDEIFERELDLMRV DNLPNMIKIATRKYLGKQNV YDIGVGEPHNFALKNGFIAS ACFN

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts142
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Fused Npu DnaE GEP loop mutant1

Entities:

Entity 1, Fused Npu DnaE GEP loop mutant 144 residues - 16493.6656 Da.

1   ALAGLUTYRALALEUSERTYRGLUTHRGLU
2   ILELEUTHRVALGLUTYRGLYLEULEUPRO
3   ILEGLYLYSILEVALGLULYSARGILEGLU
4   CYSTHRVALTYRSERVALASPASNASNGLY
5   ASNILETYRTHRGLNPROVALALAGLNTRP
6   HISASPARGGLYGLUGLNGLUVALPHEGLU
7   TYRCYSLEUGLUASPGLYSERLEUILEARG
8   ALATHRLYSASPHISLYSPHEMETTHRVAL
9   ASPGLYGLNMETLEUPROILEASPGLUILE
10   PHEGLUARGGLULEUASPLEUMETARGVAL
11   ASPASNLEUPROASNMETILELYSILEALA
12   THRARGLYSTYRLEUGLYLYSGLNASNVAL
13   TYRASPILEGLYVALGLYGLUPROHISASN
14   PHEALALEULYSASNGLYPHEILEALASER
15   ALACYSPHEASN

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