BMRB Entry 26975

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26975

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Title:
AILV Methyl Resonance Assignments for G protein alpha i3 subunit in the GDP-bound state
Deposition date:
2016-12-17
Original release date:
2017-02-21
Authors:
Toyama, Yuki; Kano, Hanaho; Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio
Citation:

Citation: Toyama, Yuki; Kano, Hanaho; Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio. "Dynamic regulation of GDP binding to G proteins revealed by magnetic field dependent NMR relaxation analyses"  Nat. Commun. 8, .-. (2017).
PubMed: 28223697

Assembly members:

Assembly members:
G_protein_alpha_subunit_i3, polymer, 359 residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G_protein_alpha_subunit_i3: GPDDHMGCTLSAEDKAAVER SKMIDRNLREDGEKAAKEVK LLLLGAGESGKSTIVKQMKI IHEDGYSEDECKQYKVVVYS NTIQSIIAIIRAMGRLKIDF GEAARADDARQLFVLAGSAE EGVMTPELAGVIKRLWRDGG VQACFSRSREYQLNDSASYY LNDLDRISQSNYIPTQQDVL RTRVKTTGIVETHFTFKDLY FKMFDVGGQRSERKKWIHCF EGVTAIIFCVALSDYDLVLA EDEEMNRMHESMKLFDSICN NKWFTETSIILFLNKKDLFE EKIKRSPLTICYPEYTGSNT YEEAAAYIQCQFEDLNRRKD TKEIYTHFTCATDTKNVQFV FDAVTDVIIKNNLKECGLY

Data sets:
Data typeCount
13C chemical shifts96
1H chemical shifts288

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1G alphai 31
2GDP2

Entities:

Entity 1, G alphai 3 359 residues - Formula weight is not available

1   GLYPROASPASPHISMETGLYCYSTHRLEU
2   SERALAGLUASPLYSALAALAVALGLUARG
3   SERLYSMETILEASPARGASNLEUARGGLU
4   ASPGLYGLULYSALAALALYSGLUVALLYS
5   LEULEULEULEUGLYALAGLYGLUSERGLY
6   LYSSERTHRILEVALLYSGLNMETLYSILE
7   ILEHISGLUASPGLYTYRSERGLUASPGLU
8   CYSLYSGLNTYRLYSVALVALVALTYRSER
9   ASNTHRILEGLNSERILEILEALAILEILE
10   ARGALAMETGLYARGLEULYSILEASPPHE
11   GLYGLUALAALAARGALAASPASPALAARG
12   GLNLEUPHEVALLEUALAGLYSERALAGLU
13   GLUGLYVALMETTHRPROGLULEUALAGLY
14   VALILELYSARGLEUTRPARGASPGLYGLY
15   VALGLNALACYSPHESERARGSERARGGLU
16   TYRGLNLEUASNASPSERALASERTYRTYR
17   LEUASNASPLEUASPARGILESERGLNSER
18   ASNTYRILEPROTHRGLNGLNASPVALLEU
19   ARGTHRARGVALLYSTHRTHRGLYILEVAL
20   GLUTHRHISPHETHRPHELYSASPLEUTYR
21   PHELYSMETPHEASPVALGLYGLYGLNARG
22   SERGLUARGLYSLYSTRPILEHISCYSPHE
23   GLUGLYVALTHRALAILEILEPHECYSVAL
24   ALALEUSERASPTYRASPLEUVALLEUALA
25   GLUASPGLUGLUMETASNARGMETHISGLU
26   SERMETLYSLEUPHEASPSERILECYSASN
27   ASNLYSTRPPHETHRGLUTHRSERILEILE
28   LEUPHELEUASNLYSLYSASPLEUPHEGLU
29   GLULYSILELYSARGSERPROLEUTHRILE
30   CYSTYRPROGLUTYRTHRGLYSERASNTHR
31   TYRGLUGLUALAALAALATYRILEGLNCYS
32   GLNPHEGLUASPLEUASNARGARGLYSASP
33   THRLYSGLUILETYRTHRHISPHETHRCYS
34   ALATHRASPTHRLYSASNVALGLNPHEVAL
35   PHEASPALAVALTHRASPVALILEILELYS
36   ASNASNLEULYSGLUCYSGLYLEUTYR

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Related Database Links:

UNP P08754
GB AAM12621.1
AlphaFold Q5TZX1