BMRB Entry 26971

Name: Chemical shifts of Lc-LTP2 in complex with DHPC
Published: 2017-04-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26971

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of Lc-LTP2 in complex with DHPC

Deposition date:

2016-12-14

Original release date:

2017-04-26

Authors:

Mineev, Konstantin; Zakhar, Shenkarev; Alexander, Arseniev

Citation:

Citation: Shenkarev, Zakhar; Melnikova, Daria; Finkina, Ekaterina; Sukhanov, Stanislav; Boldyrev, Ivan; Gizatullina, Albina; Mineev, Konstantin; Arseniev, Alexander; Ovchinnikova, Tatiana. "Ligand Binding Properties of the Lentil Lipid Transfer Protein: Molecular Insight into the Possible Mechanism of Lipid Uptake" Biochemistry 56, 1785-1796 (2017).
PubMed: 28266846

Assembly members:

Assembly members:
Ltp2, polymer, 93 residues, Formula weight is not available
1,2-dihexanoyl-sn-glycero-3-phosphocholine, non-polymer, 454.515 Da.

Natural source:

Natural source: Common Name: lentil Taxonomy ID: 3864 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: lens culinaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ltp2: AISCGAVTSDLSPCLTYLTG GPGPSPQCCGGVKKLLAAAN TTPDRQAACNCLKSAAGSIT KLNTNNAAALPGKCGVNIPY KISTTTNCNTVKF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	362
15N chemical shifts	97
1H chemical shifts	615

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ltp2	1
2	DHPC	2

Entities:

Entity 1, Ltp2 93 residues - Formula weight is not available

1

ALA

ILE

SER

CYS

GLY

ALA

VAL

THR

SER

ASP

2

LEU

SER

PRO

CYS

LEU

THR

TYR

LEU

THR

GLY

3

GLY

PRO

GLY

PRO

SER

PRO

GLN

CYS

GLY

4

GLY

VAL

LYS

LEU

ALA

ASN

5

THR

PRO

ASP

ARG

GLN

ALA

CYS

ASN

6

CYS

LEU

LYS

SER

ALA

GLY

SER

ILE

THR

7

LYS

LEU

ASN

THR

ASN

ALA

LEU

8

PRO

GLY

LYS

CYS

GLY

VAL

ASN

ILE

PRO

TYR

9

LYS

ILE

SER

THR

ASN

CYS

ASN

THR

10

VAL

LYS

PHE

Entity 2, DHPC - C20 H41 N O8 P - 454.515 Da.

1

HXG

Samples:

sample_1: Ltp2, [U-100% 13C; U-100% 15N], 1 mM; DMPC 10 mM; DHPC 15 mM

sample_conditions_1: ionic strength: 10 mM; pH: 5.7; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.4, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

UNP	A0AT29
AlphaFold	P84255