BMRB Entry 26969

Name: Backbone resonance assignments of the Pseudomonas aeruginosa major pilin PilA from strain PA14
Published: 2018-06-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26969

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone resonance assignments of the Pseudomonas aeruginosa major pilin PilA from strain PA14

Deposition date:

2016-12-07

Original release date:

2018-06-26

Authors:

Nguyen, Ylan; McNicholl, Tyler; Akimoto, Madoka; Aidoo, Francisca; Melacini, Giuseppe; Burrows, Lori

Citation:

Citation: Nguyen, Ylan; Boulton, Stephen; McNicholl, E Tyler; Akimoto, Madoka; Harvey, Hanjeong; Aidoo, Francisca; Melacini, Giuseppe; Burrows, Lori. "A Highly Dynamic Loop of the Pseudomonas aeruginosa PA14 Type IV Pilin Is Essential for Pilus Assembly." ACS Infect. Dis. 4, 936-943 (2018).
PubMed: 29342354

Assembly members:

Assembly members:
PilA, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET151

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PilA: GIDPFTIRARVTEGVGLAAS AKTLIGDSSATAGELAASAR VWNAQAGNAGATSKYVTSVR IAEATGEITVTFNATNVGNI PADSTLVFTPYVQNAAGAPT QLGASYAAGVTGSIDWGCAS ESNAVSSGPDRNMPALTAGT LPARFAPSECR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	434
15N chemical shifts	139
1H chemical shifts	139

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PilA	1

Entities:

Entity 1, PilA 151 residues - Formula weight is not available

residues 1-6 are artifacts of affinity tag cleavage

1

GLY

ILE

ASP

PRO

PHE

THR

ILE

ARG

ALA

ARG

2

VAL

THR

GLU

GLY

VAL

GLY

LEU

ALA

SER

3

ALA

LYS

THR

LEU

ILE

GLY

ASP

SER

ALA

4

THR

ALA

GLY

GLU

LEU

ALA

SER

ALA

ARG

5

VAL

TRP

ASN

ALA

GLN

ALA

GLY

ASN

ALA

GLY

6

ALA

THR

SER

LYS

TYR

VAL

THR

SER

VAL

ARG

7

ILE

ALA

GLU

ALA

THR

GLY

GLU

ILE

THR

VAL

8

THR

PHE

ASN

ALA

THR

ASN

VAL

GLY

ASN

ILE

9

PRO

ALA

ASP

SER

THR

LEU

VAL

PHE

THR

PRO

10

TYR

VAL

GLN

ASN

ALA

GLY

ALA

PRO

THR

11

GLN

LEU

GLY

ALA

SER

TYR

ALA

GLY

VAL

12

THR

GLY

SER

ILE

ASP

TRP

GLY

CYS

ALA

SER

13

GLU

SER

ASN

ALA

VAL

SER

GLY

PRO

ASP

14

ARG

ASN

MET

PRO

ALA

LEU

THR

ALA

GLY

THR

15

LEU

PRO

ALA

ARG

PHE

ALA

PRO

SER

GLU

CYS

16

ARG

Samples:

sample_1: PilA, [U-13C; U-15N], 0.5 mM; TRIS 20 mM; sodium chloride 50 mM

sample_2: PilA, [U-15N]-Leu, 0.5 mM; TRIS 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 306 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 15N NOESY-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 700 MHz
Varian INOVA 500 MHz

UNP	A0A0H2ZFW8
AlphaFold	A0A0H2ZFW8