BMRB Entry 26957
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26957
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Tunnicliffe, Richard; Lockhart-Cairns, Michael; Levy, Colin; Mould, A Paul; Jowitt, Thomas; Sito, Hilary; Baldock, Clair; Sandri-Goldin, Rozanne; Golovanov, Alexander. "The herpes viral transcription factor ICP4 forms a novel DNA recognition complex" Nucleic Acids Res. 45, 8064-8078 (2017).
PubMed: 28505309
Assembly members:
ICP4_DNA_binding_domain, polymer, 231 residues, 24367.4 Da.
Natural source: Common Name: Human herpesvirus 1 Taxonomy ID: 10298 Superkingdom: Viruses Kingdom: not available Genus/species: Simplexvirus herpesvirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 34 |
| 1H chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ICP4N | 1 |
Entities:
Entity 1, ICP4N 231 residues - 24367.4 Da.
N-terminal glycine is non-native. Remains after HRV3C protease cleavage.
| 1 | GLY | PRO | ALA | ALA | SER | ALA | GLY | ARG | ILE | GLU | ||||
| 2 | ARG | ARG | ARG | ALA | ARG | ALA | ALA | VAL | ALA | GLY | ||||
| 3 | ARG | ASP | ALA | THR | GLY | ARG | PHE | THR | ALA | GLY | ||||
| 4 | GLN | PRO | ARG | ARG | VAL | GLU | LEU | ASP | ALA | ASP | ||||
| 5 | ALA | THR | SER | GLY | ALA | PHE | TYR | ALA | ARG | TYR | ||||
| 6 | ARG | ASP | GLY | TYR | VAL | SER | GLY | GLU | PRO | TRP | ||||
| 7 | PRO | GLY | ALA | GLY | PRO | PRO | PRO | PRO | GLY | ARG | ||||
| 8 | VAL | LEU | TYR | GLY | GLY | LEU | GLY | ASP | SER | ARG | ||||
| 9 | PRO | GLY | LEU | TRP | GLY | ALA | PRO | GLU | ALA | GLU | ||||
| 10 | GLU | ALA | ARG | ARG | ARG | PHE | GLU | ALA | SER | GLY | ||||
| 11 | ALA | PRO | ALA | ALA | VAL | TRP | ALA | PRO | GLU | LEU | ||||
| 12 | GLY | ASP | ALA | ALA | GLN | GLN | TYR | ALA | LEU | ILE | ||||
| 13 | THR | ARG | LEU | LEU | TYR | THR | PRO | ASP | ALA | GLU | ||||
| 14 | ALA | MET | GLY | TRP | LEU | GLN | ASN | PRO | ARG | VAL | ||||
| 15 | VAL | PRO | GLY | ASP | VAL | ALA | LEU | ASP | GLN | ALA | ||||
| 16 | CYS | PHE | ARG | ILE | SER | GLY | ALA | ALA | ARG | ASN | ||||
| 17 | SER | SER | SER | PHE | ILE | THR | GLY | SER | VAL | ALA | ||||
| 18 | ARG | ALA | VAL | PRO | HIS | LEU | GLY | TYR | ALA | MET | ||||
| 19 | ALA | ALA | GLY | ARG | PHE | GLY | TRP | GLY | LEU | ALA | ||||
| 20 | HIS | ALA | ALA | ALA | ALA | VAL | ALA | MET | SER | ARG | ||||
| 21 | ARG | TYR | ASP | ARG | ALA | GLN | LYS | GLY | PHE | LEU | ||||
| 22 | LEU | THR | SER | LEU | ARG | ARG | ALA | TYR | ALA | PRO | ||||
| 23 | LEU | LEU | ALA | ARG | GLU | ASN | ALA | ALA | LEU | THR | ||||
| 24 | GLY |
Samples:
sample_1: ICP4 DNA binding domain, [U-100% 15N], 0.3 ± 0.01 mM; MES 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; TCEP 1 mM; MgCl2 2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.114, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks