BMRB Entry 26953

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Kruppel-like Factor 4 (KLF4) Residues 1-130
Published: 2016-11-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26953

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Kruppel-like Factor 4 (KLF4) Residues 1-130

Deposition date:

2016-11-22

Original release date:

2016-11-23

Authors:

Conroy, Brigid; Langelaan, David; Smith, Steven

Citation:

Citation: Conroy, Brigid; Weiss, Emma; Smith, Steven; Langelaan, David. "Backbone 1H, 13C, and 15N NMR resonance assignments of the Kruppel-like factor 4 activation domain" Biomol. NMR Assign. 11, 95-98 (2017).
PubMed: 28247282

Assembly members:

Assembly members:
KLF4_1-130, polymer, 132 residues, 13757.2305 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KLF4_1-130: GSMRQPPGESDMAVSDALLP SFSTFASGPAGREKTLRQAG APNNRWREELSHMKRLPPVL PGRPYDLAAATVATDLESGG AGAACGGSNLAPLPRRETEE FNDLLDLDFILSNSLTHPPE SVAATVSSSASA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	379
15N chemical shifts	116
1H chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KLF4 1-130	1

Entities:

Entity 1, KLF4 1-130 132 residues - 13757.2305 Da.

A Gly-Ser cloning artifact has been added to the N-terminus of the KLF4 1-130 fragment.

1

GLY

SER

MET

ARG

GLN

PRO

GLY

GLU

SER

2

ASP

MET

ALA

VAL

SER

ASP

ALA

LEU

PRO

3

SER

PHE

SER

THR

PHE

ALA

SER

GLY

PRO

ALA

4

GLY

ARG

GLU

LYS

THR

LEU

ARG

GLN

ALA

GLY

5

ALA

PRO

ASN

ARG

TRP

ARG

GLU

LEU

6

SER

HIS

MET

LYS

ARG

LEU

PRO

VAL

LEU

7

PRO

GLY

ARG

PRO

TYR

ASP

LEU

ALA

8

THR

VAL

ALA

THR

ASP

LEU

GLU

SER

GLY

9

ALA

GLY

ALA

CYS

GLY

SER

ASN

LEU

10

ALA

PRO

LEU

PRO

ARG

GLU

THR

GLU

11

PHE

ASN

ASP

LEU

ASP

LEU

ASP

PHE

ILE

12

LEU

SER

ASN

SER

LEU

THR

HIS

PRO

GLU

13

SER

VAL

ALA

THR

VAL

SER

ALA

14

SER

ALA

Samples:

sample_1: MES 20 mM; DTT 5 mM; KLF4 1-130, [U-100% 13C; U-100% 15N], 1.3 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking, processing

NMRView v8.0.3, Johnson, One Moon Scientific - collection

NMR spectrometers:

Varian INOVA 600 MHz

UNP	O43474
AlphaFold	Q9UNP3