BMRB Entry 26949

Name: Early Response to Dehydration 10 (ERD10) from Arabidopsis thaliana
Published: 2017-02-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26949

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Early Response to Dehydration 10 (ERD10) from Arabidopsis thaliana

Deposition date:

2016-11-21

Original release date:

2017-02-20

Authors:

Cedeno, Cesyen; Zerko, Szymon; Tompa, Peter; Kosminzki, Wiktor

Citation:

Citation: Cedeno, Cesyen; Zerko, Szymon; Tompa, Peter; Kosminzki, Wiktor. "1H, 15N,13C resonance assignment of plant dehydrin Early Response to Dehydration 10 (ERD10)" Biomol. NMR Assign. 11, 127-131 (2017).
PubMed: 28275980

Assembly members:

Assembly members:
ERD10, polymer, 260 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ERD10: MAEEYKNTVPEQETPKVATE ESSAPEIKERGMFDFLKKKE EVKPQETTTLASEFEHKTQI SEPESFVAKHEEEEHKPTLL EQLHQKHEEEEENKPSLLDK LHRSNSSSSSSSDEEGEDGE KKKKEKKKKIVEGDHVKTVE EENQGVMDRIKEKFPLGEKP GGDDVPVVTTMPAPHSVEDH KPEEEEKKGFMDKIKEKLPG HSKKPEDSQVVNTTPLVETA TPIADIPEEKKGFMDKIKEK LPGYHAKTTGEEEKKEKVSD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	661
15N chemical shifts	237
1H chemical shifts	836

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ERD10	1

Entities:

Entity 1, ERD10 260 residues - Formula weight is not available

1	MET	ALA	GLU	GLU	TYR	LYS	ASN	THR	VAL	PRO
2	GLU	GLN	GLU	THR	PRO	LYS	VAL	ALA	THR	GLU
3	GLU	SER	SER	ALA	PRO	GLU	ILE	LYS	GLU	ARG
4	GLY	MET	PHE	ASP	PHE	LEU	LYS	LYS	LYS	GLU
5	GLU	VAL	LYS	PRO	GLN	GLU	THR	THR	THR	LEU
6	ALA	SER	GLU	PHE	GLU	HIS	LYS	THR	GLN	ILE
7	SER	GLU	PRO	GLU	SER	PHE	VAL	ALA	LYS	HIS
8	GLU	GLU	GLU	GLU	HIS	LYS	PRO	THR	LEU	LEU
9	GLU	GLN	LEU	HIS	GLN	LYS	HIS	GLU	GLU	GLU
10	GLU	GLU	ASN	LYS	PRO	SER	LEU	LEU	ASP	LYS
11	LEU	HIS	ARG	SER	ASN	SER	SER	SER	SER	SER
12	SER	SER	ASP	GLU	GLU	GLY	GLU	ASP	GLY	GLU
13	LYS	LYS	LYS	LYS	GLU	LYS	LYS	LYS	LYS	ILE
14	VAL	GLU	GLY	ASP	HIS	VAL	LYS	THR	VAL	GLU
15	GLU	GLU	ASN	GLN	GLY	VAL	MET	ASP	ARG	ILE
16	LYS	GLU	LYS	PHE	PRO	LEU	GLY	GLU	LYS	PRO
17	GLY	GLY	ASP	ASP	VAL	PRO	VAL	VAL	THR	THR
18	MET	PRO	ALA	PRO	HIS	SER	VAL	GLU	ASP	HIS
19	LYS	PRO	GLU	GLU	GLU	GLU	LYS	LYS	GLY	PHE
20	MET	ASP	LYS	ILE	LYS	GLU	LYS	LEU	PRO	GLY
21	HIS	SER	LYS	LYS	PRO	GLU	ASP	SER	GLN	VAL
22	VAL	ASN	THR	THR	PRO	LEU	VAL	GLU	THR	ALA
23	THR	PRO	ILE	ALA	ASP	ILE	PRO	GLU	GLU	LYS
24	LYS	GLY	PHE	MET	ASP	LYS	ILE	LYS	GLU	LYS
25	LEU	PRO	GLY	TYR	HIS	ALA	LYS	THR	THR	GLY
26	GLU	GLU	GLU	LYS	LYS	GLU	LYS	VAL	SER	ASP

Samples:

sample_1: ERD10, [U-100% 13C; U-100% 15N], 250 uM; MES 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
5D (HACA)CON(CA)CONH	sample_1	isotropic	sample_conditions_1
5D HN(CA)CONH	sample_1	isotropic	sample_conditions_1
5D (H)NCO(NCA)CONH	sample_1	isotropic	sample_conditions_1
5D HabCabCONH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Agilent Uniform NMR System 800 MHz

1	MET	ALA	GLU	GLU	TYR	LYS	ASN	THR	VAL	PRO
2	GLU	GLN	GLU	THR	PRO	LYS	VAL	ALA	THR	GLU
3	GLU	SER	SER	ALA	PRO	GLU	ILE	LYS	GLU	ARG
4	GLY	MET	PHE	ASP	PHE	LEU	LYS	LYS	LYS	GLU
5	GLU	VAL	LYS	PRO	GLN	GLU	THR	THR	THR	LEU
6	ALA	SER	GLU	PHE	GLU	HIS	LYS	THR	GLN	ILE
7	SER	GLU	PRO	GLU	SER	PHE	VAL	ALA	LYS	HIS
8	GLU	GLU	GLU	GLU	HIS	LYS	PRO	THR	LEU	LEU
9	GLU	GLN	LEU	HIS	GLN	LYS	HIS	GLU	GLU	GLU
10	GLU	GLU	ASN	LYS	PRO	SER	LEU	LEU	ASP	LYS
11	LEU	HIS	ARG	SER	ASN	SER	SER	SER	SER	SER
12	SER	SER	ASP	GLU	GLU	GLY	GLU	ASP	GLY	GLU
13	LYS	LYS	LYS	LYS	GLU	LYS	LYS	LYS	LYS	ILE
14	VAL	GLU	GLY	ASP	HIS	VAL	LYS	THR	VAL	GLU
15	GLU	GLU	ASN	GLN	GLY	VAL	MET	ASP	ARG	ILE
16	LYS	GLU	LYS	PHE	PRO	LEU	GLY	GLU	LYS	PRO
17	GLY	GLY	ASP	ASP	VAL	PRO	VAL	VAL	THR	THR
18	MET	PRO	ALA	PRO	HIS	SER	VAL	GLU	ASP	HIS
19	LYS	PRO	GLU	GLU	GLU	GLU	LYS	LYS	GLY	PHE
20	MET	ASP	LYS	ILE	LYS	GLU	LYS	LEU	PRO	GLY
21	HIS	SER	LYS	LYS	PRO	GLU	ASP	SER	GLN	VAL
22	VAL	ASN	THR	THR	PRO	LEU	VAL	GLU	THR	ALA
23	THR	PRO	ILE	ALA	ASP	ILE	PRO	GLU	GLU	LYS
24	LYS	GLY	PHE	MET	ASP	LYS	ILE	LYS	GLU	LYS
25	LEU	PRO	GLY	TYR	HIS	ALA	LYS	THR	THR	GLY
26	GLU	GLU	GLU	LYS	LYS	GLU	LYS	VAL	SER	ASP

1	MET	ALA	GLU	GLU	TYR	LYS	ASN	THR	VAL	PRO
2	GLU	GLN	GLU	THR	PRO	LYS	VAL	ALA	THR	GLU
3	GLU	SER	SER	ALA	PRO	GLU	ILE	LYS	GLU	ARG
4	GLY	MET	PHE	ASP	PHE	LEU	LYS	LYS	LYS	GLU
5	GLU	VAL	LYS	PRO	GLN	GLU	THR	THR	THR	LEU
6	ALA	SER	GLU	PHE	GLU	HIS	LYS	THR	GLN	ILE
7	SER	GLU	PRO	GLU	SER	PHE	VAL	ALA	LYS	HIS
8	GLU	GLU	GLU	GLU	HIS	LYS	PRO	THR	LEU	LEU
9	GLU	GLN	LEU	HIS	GLN	LYS	HIS	GLU	GLU	GLU
10	GLU	GLU	ASN	LYS	PRO	SER	LEU	LEU	ASP	LYS
11	LEU	HIS	ARG	SER	ASN	SER	SER	SER	SER	SER
12	SER	SER	ASP	GLU	GLU	GLY	GLU	ASP	GLY	GLU
13	LYS	LYS	LYS	LYS	GLU	LYS	LYS	LYS	LYS	ILE
14	VAL	GLU	GLY	ASP	HIS	VAL	LYS	THR	VAL	GLU
15	GLU	GLU	ASN	GLN	GLY	VAL	MET	ASP	ARG	ILE
16	LYS	GLU	LYS	PHE	PRO	LEU	GLY	GLU	LYS	PRO
17	GLY	GLY	ASP	ASP	VAL	PRO	VAL	VAL	THR	THR
18	MET	PRO	ALA	PRO	HIS	SER	VAL	GLU	ASP	HIS
19	LYS	PRO	GLU	GLU	GLU	GLU	LYS	LYS	GLY	PHE
20	MET	ASP	LYS	ILE	LYS	GLU	LYS	LEU	PRO	GLY
21	HIS	SER	LYS	LYS	PRO	GLU	ASP	SER	GLN	VAL
22	VAL	ASN	THR	THR	PRO	LEU	VAL	GLU	THR	ALA
23	THR	PRO	ILE	ALA	ASP	ILE	PRO	GLU	GLU	LYS
24	LYS	GLY	PHE	MET	ASP	LYS	ILE	LYS	GLU	LYS
25	LEU	PRO	GLY	TYR	HIS	ALA	LYS	THR	THR	GLY
26	GLU	GLU	GLU	LYS	LYS	GLU	LYS	VAL	SER	ASP