BMRB Entry 26924

Name: 13C and 15N Chemical Shift Assignments for Mouse Y145Stop Prion Protein Amyloid Fibrils
Published: 2017-02-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26924

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C and 15N Chemical Shift Assignments for Mouse Y145Stop Prion Protein Amyloid Fibrils

Deposition date:

2016-10-26

Original release date:

2017-02-15

Authors:

Theint, Theint; Nadaud, Philippe; Surewicz, Krystyna; Surewicz, Witold; Jaroniec, Christopher

Citation:

Citation: Theint, Theint; Nadaud, Philippe; Surewicz, Krystyna; Surewicz, Witold; Jaroniec, Christopher. "13C and 15N Chemical Shift Assignments of Mammalian Y145Stop Prion Protein Amyloid Fibrils" Biomol. NMR Assign. 11, 75-80 (2017).
PubMed: 28004358

Assembly members:

Assembly members:
moPrP23-144, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSETB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
moPrP23-144: GSDPKKRPKPGGWNTGGSRY PGQGSPGGNRYPPQGGTWGQ PHGGGWGQPHGGSWGQPHGG SWGQPHGGGWGQGGGTHNQW NKPSKPKTNLKHVAGAAAAG AVVGGLGGYMLGSAMSRPMI HFGND

Data sets:

assigned_chemical_shifts
- moPrP23-144_amyloid

Data type	Count
13C chemical shifts	83
15N chemical shifts	30

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	moPrP23-144	1

Entities:

Entity 1, moPrP23-144 125 residues - Formula weight is not available

The four N-terminal residues (GSDP) correspond to a non-native thrombin cleavage site used for protein purification.

1

GLY

SER

ASP

PRO

LYS

ARG

PRO

LYS

PRO

2

GLY

TRP

ASN

THR

GLY

SER

ARG

TYR

3

PRO

GLY

GLN

GLY

SER

PRO

GLY

ASN

ARG

4

TYR

PRO

GLN

GLY

THR

TRP

GLY

GLN

5

PRO

HIS

GLY

TRP

GLY

GLN

PRO

HIS

6

GLY

SER

TRP

GLY

GLN

PRO

HIS

GLY

7

SER

TRP

GLY

GLN

PRO

HIS

GLY

TRP

8

GLY

GLN

GLY

THR

HIS

ASN

GLN

TRP

9

ASN

LYS

PRO

SER

LYS

PRO

LYS

THR

ASN

LEU

10

LYS

HIS

VAL

ALA

GLY

ALA

GLY

11

ALA

VAL

GLY

LEU

GLY

TYR

MET

12

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

MET

ILE

13

HIS

PHE

GLY

ASN

ASP

Samples:

sample_1: mouse PrP23-144, [U-100% 13C; U-100% 15N], 20 mg

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NCA	sample_1	solid	sample_conditions_1
3D NCACX	sample_1	solid	sample_conditions_1
3D NCOCX	sample_1	solid	sample_conditions_1
3D CONCA	sample_1	solid	sample_conditions_1
3D NCACX	sample_1	solid	sample_conditions_1

Software:

VnmrJ v2.2C, Varian - collection

SPARKY, Goddard - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS-N, Shen and Bax - secondary structure analysis

nmrglue, Helmus, Jaroniec - processing

NMR spectrometers:

Varian VNMRS 500 MHz