BMRB Entry 26923

Name: Backbone and Ile, Leu, Val Methyl Chemical Shift Assignments for Rhizopus microsporus var. chinensis lipase
Published: 2018-02-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26923

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and Ile, Leu, Val Methyl Chemical Shift Assignments for Rhizopus microsporus var. chinensis lipase

Deposition date:

2016-10-24

Original release date:

2018-02-09

Authors:

Zhang, Meng; Yu, Xiao-Wei; G.V.T., Swapna; Liu, Gaohua; Xiao, Rong; Xu, Yan; Montelione, Gaetano

Citation:

Citation: Zhang, Meng; Yu, Xiao-Wei; G.V.T., Swapna; Liu, Gaohua; Xiao, Rong; Xu, Yan; Montelione, Gaetano. "Backbone and Ile-delta1, Leu, Val methyl 1H, 15N, and 13C, chemical shift assignments for Rhizopus chinensis lipase" Biomol. NMR Assign. 12, 63-68 (2018).
PubMed: 28929427

Assembly members:

Assembly members:
RCL, polymer, 296 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rhizopus microsporus Taxonomy ID: 58291 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Rhizopus microsporus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: MBP3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RCL: DTETVGGMTLDLPENPPPIP ATSTAPSSDSGEVVTATAAQ IKELTNYAGVAATAYCRSVV PGTKWDCKQCLKYVPDGKLI KTFTSLLTDTNGFILRSDAQ KTIYVTFRGTNSFRSAITDM VFTFTDYSPVKGAKVHAGFL SSYNQVVKDYFPVVQDQLTA YPDYKVIVTGHSLGGAQALL AGMDLYQREKRLSPKNLSIY TVGCPRVGNNAFAYYVDSTG IPFHRTVHKRDIVPHVPPQA FGYLHPGVESWIKEDPADVQ ICTSNIETKQCSNSIVPFTS IADHLTYFGINEGSCL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	554
15N chemical shifts	228
1H chemical shifts	477

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RCL	1

Entities:

Entity 1, RCL 296 residues - Formula weight is not available

1

ASP

THR

GLU

THR

VAL

GLY

MET

THR

LEU

2

ASP

LEU

PRO

GLU

ASN

PRO

ILE

PRO

3

ALA

THR

SER

THR

ALA

PRO

SER

ASP

SER

4

GLY

GLU

VAL

THR

ALA

THR

ALA

GLN

5

ILE

LYS

GLU

LEU

THR

ASN

TYR

ALA

GLY

VAL

6

ALA

THR

ALA

TYR

CYS

ARG

SER

VAL

7

PRO

GLY

THR

LYS

TRP

ASP

CYS

LYS

GLN

CYS

8

LEU

LYS

TYR

VAL

PRO

ASP

GLY

LYS

LEU

ILE

9

LYS

THR

PHE

THR

SER

LEU

THR

ASP

THR

10

ASN

GLY

PHE

ILE

LEU

ARG

SER

ASP

ALA

GLN

11

LYS

THR

ILE

TYR

VAL

THR

PHE

ARG

GLY

THR

12

ASN

SER

PHE

ARG

SER

ALA

ILE

THR

ASP

MET

13

VAL

PHE

THR

PHE

THR

ASP

TYR

SER

PRO

VAL

14

LYS

GLY

ALA

LYS

VAL

HIS

ALA

GLY

PHE

LEU

15

SER

TYR

ASN

GLN

VAL

LYS

ASP

TYR

16

PHE

PRO

VAL

GLN

ASP

GLN

LEU

THR

ALA

17

TYR

PRO

ASP

TYR

LYS

VAL

ILE

VAL

THR

GLY

18

HIS

SER

LEU

GLY

ALA

GLN

ALA

LEU

19

ALA

GLY

MET

ASP

LEU

TYR

GLN

ARG

GLU

LYS

20

ARG

LEU

SER

PRO

LYS

ASN

LEU

SER

ILE

TYR

21

THR

VAL

GLY

CYS

PRO

ARG

VAL

GLY

ASN

22

ALA

PHE

ALA

TYR

VAL

ASP

SER

THR

GLY

23

ILE

PRO

PHE

HIS

ARG

THR

VAL

HIS

LYS

ARG

24

ASP

ILE

VAL

PRO

HIS

VAL

PRO

GLN

ALA

25

PHE

GLY

TYR

LEU

HIS

PRO

GLY

VAL

GLU

SER

26

TRP

ILE

LYS

GLU

ASP

PRO

ALA

ASP

VAL

GLN

27

ILE

CYS

THR

SER

ASN

ILE

GLU

THR

LYS

GLN

28

CYS

SER

ASN

SER

ILE

VAL

PRO

PHE

THR

SER

29

ILE

ALA

ASP

HIS

LEU

THR

TYR

PHE

GLY

ILE

30

ASN

GLU

GLY

SER

CYS

LEU

Samples:

sample_1: RCL, [U-13C; U-15N; U-2H], 0.12 mM; DMSO, [U-99% 2H], 2%; DSS 50 uM; D2O, [U-99% 2H], 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

TOPSPIN, Bruker Biospin - collection

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz