BMRB Entry 26914
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26914
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Citation: Gahloth, Deepankor; Levy, Colin; Heaven, Graham; Stefani, Flavia; Wunderley, Lydia; Mold, Paul; Cliff, Matthew; Bella, Jordi; Fielding, Alistair; Woodman, Philip; Tabernero, Lydia. "Structural Basis for Selective Interaction between the ESCRT Regulator HD-PTP and UBAP1" Structure 24, 2115-2126 (2016).
PubMed: 27839950
Assembly members:
UBAP1, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
UBAP1: SNIKSLSFPKLDSDDSNQKT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 48 |
| 1H chemical shifts | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBAP1 | 1 |
Entities:
Entity 1, UBAP1 20 residues - Formula weight is not available
| 1 | SER | ASN | ILE | LYS | SER | LEU | SER | PHE | PRO | LYS | |
| 2 | LEU | ASP | SER | ASP | ASP | SER | ASN | GLN | LYS | THR |
Samples:
sample_1: UBAP1 3 mg/mL; D2O, [U-100% 2H], 100 v/v; sodium phosphate 50 mM; sodium azide 1 mM; TSP 0.01 v/v
sample_2: UBAP1 3 mg/mL; D2O, [U-2H], 100 v/v; sodium phosphate 50 mM; sodium azide 3 mM; TSP 0.01 v/v
sample_conditions_1: ionic strength: 50 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - collection, processing
CCPN_Analysis, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz