BMRB Entry 26907

Name: Chemical shift assignments of a hydrophobin from Phanerochaete carnosa
Published: 2017-04-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26907

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments of a hydrophobin from Phanerochaete carnosa

Deposition date:

2016-09-23

Original release date:

2017-04-26

Authors:

Langelaan, David; Smith, Steven

Citation:

Citation: Gandier, Julie-Anne; Langelaan, David; Won, Amy; O'Donnell, Kylie; Grondin, Julie; Spencer, Holly; Wong, Philip; Tillier, Elisabeth; Yip, Christopher; Smith, Steven; Master, Emma. "Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision" Sci. Rep. 7, 45863-45863 (2017).
PubMed: 28393921

Assembly members:

Assembly members:
hfb4, polymer, 126 residues, 12301.7867 Da.

Natural source:

Natural source: Common Name: Phanerochaete carnosa Taxonomy ID: 231932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phanerochaete carnosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hfb4: KAMADIGSTPNPENAKRWAT TTTPQAPTTTVTVTETATAS SVPASDCSTGSVQCCNTVTS ASDPTAAGILGLLGVVVQGV DVLVGLTCDPITVVGAGGGS CSAQAVCCQDNSYGSLISIG CIPVTL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	311
15N chemical shifts	79
1H chemical shifts	475

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hfb4	1

Entities:

Entity 1, hfb4 126 residues - 12301.7867 Da.

1

LYS

ALA

MET

ALA

ASP

ILE

GLY

SER

THR

PRO

2

ASN

PRO

GLU

ASN

ALA

LYS

ARG

TRP

ALA

THR

3

THR

PRO

GLN

ALA

PRO

THR

4

VAL

THR

VAL

THR

GLU

THR

ALA

THR

ALA

SER

5

SER

VAL

PRO

ALA

SER

ASP

CYS

SER

THR

GLY

6

SER

VAL

GLN

CYS

ASN

THR

VAL

THR

SER

7

ALA

SER

ASP

PRO

THR

ALA

GLY

ILE

LEU

8

GLY

LEU

GLY

VAL

GLN

GLY

VAL

9

ASP

VAL

LEU

VAL

GLY

LEU

THR

CYS

ASP

PRO

10

ILE

THR

VAL

GLY

ALA

GLY

SER

11

CYS

SER

ALA

GLN

ALA

VAL

CYS

GLN

ASP

12

ASN

SER

TYR

GLY

SER

LEU

ILE

SER

ILE

GLY

13

CYS

ILE

PRO

VAL

THR

LEU

Samples:

sample_1: PcaHyd1, [U-99% 13C; U-99% 15N], 1 mM; TRIS 25 mM; sodium chloride 150 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking, processing

VNMRJ, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks