BMRB Entry 26883

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26883

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Title:
HIV-1 Capsid-SP1 in immature virus-like particles
Deposition date:
2016-08-29
Original release date:
2017-02-16
Authors:
Ganser-Pornillos, Barbie; Zadrozny, Kaneil; Yeager, Mark
Citation:

Citation: Bayro, Marvin; Ganser-Pornillos, Barbie; Zadrozny, Kaneil; Yeager, Mark; Tycko, Robert. "Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles"  J. Am. Chem. Soc. 138, 12029-12032 (2016).
PubMed: 27593947

Assembly members:

Assembly members:
dMA-CA-SP1-NC, polymer, 348 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: viruses   Taxonomy ID: 12721   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dMA-CA-SP1-NC: MGARASVLSGGELDKALDKI EEEQNKSKKKAQQAAADTGN NSQVSQNYPIVQNLQGQMVH QAISPRTLNAWVKVVEEKAF SPEVIPMFSALSEGATPQDL NTMLNTVGGHQAAMQMLKET INEEAAEWDRLHPVHAGPIA PGQMREPRGSDIAGTTSTLQ EQIGWMTHNPPIPVGEIYKR WIILGLNKIVRMYSPTSILD IRQGPKEPFRDYVDRFYKTL RAEQASQEVKNWMTETLLVQ NANPDCKTILKALGPGATLE EMMTACQGVGGPGHKARVLA EAMSQVCNPATIMIQKGNFR NQRKTVKCFNCGKEGHIAKN CRAPRKKGCWKCGKEGHQMK DCTERQAN

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1virus-like particles1

Entities:

Entity 1, virus-like particles 348 residues - Formula weight is not available

1   METGLYALAARGALASERVALLEUSERGLY
2   GLYGLULEUASPLYSALALEUASPLYSILE
3   GLUGLUGLUGLNASNLYSSERLYSLYSLYS
4   ALAGLNGLNALAALAALAASPTHRGLYASN
5   ASNSERGLNVALSERGLNASNTYRPROILE
6   VALGLNASNLEUGLNGLYGLNMETVALHIS
7   GLNALAILESERPROARGTHRLEUASNALA
8   TRPVALLYSVALVALGLUGLULYSALAPHE
9   SERPROGLUVALILEPROMETPHESERALA
10   LEUSERGLUGLYALATHRPROGLNASPLEU
11   ASNTHRMETLEUASNTHRVALGLYGLYHIS
12   GLNALAALAMETGLNMETLEULYSGLUTHR
13   ILEASNGLUGLUALAALAGLUTRPASPARG
14   LEUHISPROVALHISALAGLYPROILEALA
15   PROGLYGLNMETARGGLUPROARGGLYSER
16   ASPILEALAGLYTHRTHRSERTHRLEUGLN
17   GLUGLNILEGLYTRPMETTHRHISASNPRO
18   PROILEPROVALGLYGLUILETYRLYSARG
19   TRPILEILELEUGLYLEUASNLYSILEVAL
20   ARGMETTYRSERPROTHRSERILELEUASP
21   ILEARGGLNGLYPROLYSGLUPROPHEARG
22   ASPTYRVALASPARGPHETYRLYSTHRLEU
23   ARGALAGLUGLNALASERGLNGLUVALLYS
24   ASNTRPMETTHRGLUTHRLEULEUVALGLN
25   ASNALAASNPROASPCYSLYSTHRILELEU
26   LYSALALEUGLYPROGLYALATHRLEUGLU
27   GLUMETMETTHRALACYSGLNGLYVALGLY
28   GLYPROGLYHISLYSALAARGVALLEUALA
29   GLUALAMETSERGLNVALCYSASNPROALA
30   THRILEMETILEGLNLYSGLYASNPHEARG
31   ASNGLNARGLYSTHRVALLYSCYSPHEASN
32   CYSGLYLYSGLUGLYHISILEALALYSASN
33   CYSARGALAPROARGLYSLYSGLYCYSTRP
34   LYSCYSGLYLYSGLUGLYHISGLNMETLYS
35   ASPCYSTHRGLUARGGLNALAASN

Samples:

sample_1: dMA-CA-SP1-NC, [U-98% 13C; U-98% 15N], 13 mM

sample_conditions_1: ionic strength: 1 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CONCAsample_1isotropicsample_conditions_1
2D CCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Infinity 600 MHz