BMRB Entry 26881

Name: Backbone 1H, 15N assignment for LC3B
Published: 2017-02-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26881

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N assignment for LC3B

Deposition date:

2016-08-26

Original release date:

2017-02-16

Authors:

Varani, Luca; Simonelli, Luca

Citation:

Citation: Fumagalli, Fiorenza; Noack, Julia; Bergmann, Timothy; Cebollero, Eduardo; Pisoni, Giorgia Brambilla; Fasana, Elisa; Fregno, Ilaria; Galli, Carmela; Loi, Marisa; Solda, Tatiana; D'Antuono, Rocco; Raimondi, Andrea; Jung, Martin; Melnyk, Armin; Schorr, Stefan; Schreiber, Anne; Simonelli, Luca; Varani, Luca; Wilson-Zbinden, Caroline; Zerbe, Oliver; Hofmann, Kay; Peter, Matthias; Quadroni, Manfredo; Zimmermann, Richard; Molinari, Maurizio. "Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery" Nat. Cell Biol. 18, 1173-1184 (2016).
PubMed: 27749824

Assembly members:

Assembly members:
Microtubule-associated_proteins_1A/1B_light_chain_3B, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Microtubule-associated_proteins_1A/1B_light_chain_3B: MPSEKTFKQRRTFEQRVEDV RLIREQHPTKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM SELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYE SEKDEDGFLYMVYASQETFG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	101
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LC3B monomer	1

Entities:

Entity 1, LC3B monomer 120 residues - Formula weight is not available

1	MET	PRO	SER	GLU	LYS	THR	PHE	LYS	GLN	ARG
2	ARG	THR	PHE	GLU	GLN	ARG	VAL	GLU	ASP	VAL
3	ARG	LEU	ILE	ARG	GLU	GLN	HIS	PRO	THR	LYS
4	ILE	PRO	VAL	ILE	ILE	GLU	ARG	TYR	LYS	GLY
5	GLU	LYS	GLN	LEU	PRO	VAL	LEU	ASP	LYS	THR
6	LYS	PHE	LEU	VAL	PRO	ASP	HIS	VAL	ASN	MET
7	SER	GLU	LEU	ILE	LYS	ILE	ILE	ARG	ARG	ARG
8	LEU	GLN	LEU	ASN	ALA	ASN	GLN	ALA	PHE	PHE
9	LEU	LEU	VAL	ASN	GLY	HIS	SER	MET	VAL	SER
10	VAL	SER	THR	PRO	ILE	SER	GLU	VAL	TYR	GLU
11	SER	GLU	LYS	ASP	GLU	ASP	GLY	PHE	LEU	TYR
12	MET	VAL	TYR	ALA	SER	GLN	GLU	THR	PHE	GLY

Samples:

sample_1: Microtubule-associated proteins 1A/1B light chain 3B, [U-100% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	PRO	SER	GLU	LYS	THR	PHE	LYS	GLN	ARG
2	ARG	THR	PHE	GLU	GLN	ARG	VAL	GLU	ASP	VAL
3	ARG	LEU	ILE	ARG	GLU	GLN	HIS	PRO	THR	LYS
4	ILE	PRO	VAL	ILE	ILE	GLU	ARG	TYR	LYS	GLY
5	GLU	LYS	GLN	LEU	PRO	VAL	LEU	ASP	LYS	THR
6	LYS	PHE	LEU	VAL	PRO	ASP	HIS	VAL	ASN	MET
7	SER	GLU	LEU	ILE	LYS	ILE	ILE	ARG	ARG	ARG
8	LEU	GLN	LEU	ASN	ALA	ASN	GLN	ALA	PHE	PHE
9	LEU	LEU	VAL	ASN	GLY	HIS	SER	MET	VAL	SER
10	VAL	SER	THR	PRO	ILE	SER	GLU	VAL	TYR	GLU
11	SER	GLU	LYS	ASP	GLU	ASP	GLY	PHE	LEU	TYR
12	MET	VAL	TYR	ALA	SER	GLN	GLU	THR	PHE	GLY

1	MET	PRO	SER	GLU	LYS	THR	PHE	LYS	GLN	ARG
2	ARG	THR	PHE	GLU	GLN	ARG	VAL	GLU	ASP	VAL
3	ARG	LEU	ILE	ARG	GLU	GLN	HIS	PRO	THR	LYS
4	ILE	PRO	VAL	ILE	ILE	GLU	ARG	TYR	LYS	GLY
5	GLU	LYS	GLN	LEU	PRO	VAL	LEU	ASP	LYS	THR
6	LYS	PHE	LEU	VAL	PRO	ASP	HIS	VAL	ASN	MET
7	SER	GLU	LEU	ILE	LYS	ILE	ILE	ARG	ARG	ARG
8	LEU	GLN	LEU	ASN	ALA	ASN	GLN	ALA	PHE	PHE
9	LEU	LEU	VAL	ASN	GLY	HIS	SER	MET	VAL	SER
10	VAL	SER	THR	PRO	ILE	SER	GLU	VAL	TYR	GLU
11	SER	GLU	LYS	ASP	GLU	ASP	GLY	PHE	LEU	TYR
12	MET	VAL	TYR	ALA	SER	GLN	GLU	THR	PHE	GLY

1	MET	PRO	SER	GLU	LYS	THR	PHE	LYS	GLN	ARG
2	ARG	THR	PHE	GLU	GLN	ARG	VAL	GLU	ASP	VAL
3	ARG	LEU	ILE	ARG	GLU	GLN	HIS	PRO	THR	LYS
4	ILE	PRO	VAL	ILE	ILE	GLU	ARG	TYR	LYS	GLY
5	GLU	LYS	GLN	LEU	PRO	VAL	LEU	ASP	LYS	THR
6	LYS	PHE	LEU	VAL	PRO	ASP	HIS	VAL	ASN	MET
7	SER	GLU	LEU	ILE	LYS	ILE	ILE	ARG	ARG	ARG
8	LEU	GLN	LEU	ASN	ALA	ASN	GLN	ALA	PHE	PHE
9	LEU	LEU	VAL	ASN	GLY	HIS	SER	MET	VAL	SER
10	VAL	SER	THR	PRO	ILE	SER	GLU	VAL	TYR	GLU
11	SER	GLU	LYS	ASP	GLU	ASP	GLY	PHE	LEU	TYR
12	MET	VAL	TYR	ALA	SER	GLN	GLU	THR	PHE	GLY