BMRB Entry 26871

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26871

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Title:
Backbone 1H, 13C, and 15N chemical shift assignment for Dpo4 binary complex with DNA
Deposition date:
2016-08-08
Original release date:
2017-02-16
Authors:
Lee, Eunjeong
Citation:

Citation: Lee, Eunjeong; Fowler, Jason; Suo, Zucai; Wu, Zhengrong. "Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA"  Biomol. NMR Assign. 11, 39-43 (2017).
PubMed: 27738883

Assembly members:

Assembly members:
Y-family_DNA_polymerase_IV_Dpo4, polymer, 360 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Sulfolobus solfataricus   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Y-family_DNA_polymerase_IV_Dpo4: MIVLFVDFDYFYAQVEEVLN PSLKGKPVVVCVFSGRFEDS GAVATANYEARKFGVKAGIP IVEAKKILPNAVYLPMRKEV YQQVSSRIMNLLREYSEKIE IASIDEAYLDISDKVRDYRE AYNLGLEIKNKILEKEKITV TVGISKNKVFAKIAADMAKP NGIKVIDDEEVKRLIRELDI ADVPGIGNITAEKLKKLGIN KLVDTLSIEFDKLKGMIGEA KAKYLISLARDEYNEPIRTR VRKSIGRIVTMKRNSRNLEE IKPYLFRAIEESYYKLDKRI PKAIHVVAVTEDLDIVSRGR TFPHGISKETAYSESVKLLQ KILEEDERKIRRIGVRFSKF IEAIGLDKFFDTLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts927
15N chemical shifts294
1H chemical shifts406

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dpo4 Binary core and little finger domain1

Entities:

Entity 1, Dpo4 Binary core and little finger domain 360 residues - Formula weight is not available

1   METILEVALLEUPHEVALASPPHEASPTYR
2   PHETYRALAGLNVALGLUGLUVALLEUASN
3   PROSERLEULYSGLYLYSPROVALVALVAL
4   CYSVALPHESERGLYARGPHEGLUASPSER
5   GLYALAVALALATHRALAASNTYRGLUALA
6   ARGLYSPHEGLYVALLYSALAGLYILEPRO
7   ILEVALGLUALALYSLYSILELEUPROASN
8   ALAVALTYRLEUPROMETARGLYSGLUVAL
9   TYRGLNGLNVALSERSERARGILEMETASN
10   LEULEUARGGLUTYRSERGLULYSILEGLU
11   ILEALASERILEASPGLUALATYRLEUASP
12   ILESERASPLYSVALARGASPTYRARGGLU
13   ALATYRASNLEUGLYLEUGLUILELYSASN
14   LYSILELEUGLULYSGLULYSILETHRVAL
15   THRVALGLYILESERLYSASNLYSVALPHE
16   ALALYSILEALAALAASPMETALALYSPRO
17   ASNGLYILELYSVALILEASPASPGLUGLU
18   VALLYSARGLEUILEARGGLULEUASPILE
19   ALAASPVALPROGLYILEGLYASNILETHR
20   ALAGLULYSLEULYSLYSLEUGLYILEASN
21   LYSLEUVALASPTHRLEUSERILEGLUPHE
22   ASPLYSLEULYSGLYMETILEGLYGLUALA
23   LYSALALYSTYRLEUILESERLEUALAARG
24   ASPGLUTYRASNGLUPROILEARGTHRARG
25   VALARGLYSSERILEGLYARGILEVALTHR
26   METLYSARGASNSERARGASNLEUGLUGLU
27   ILELYSPROTYRLEUPHEARGALAILEGLU
28   GLUSERTYRTYRLYSLEUASPLYSARGILE
29   PROLYSALAILEHISVALVALALAVALTHR
30   GLUASPLEUASPILEVALSERARGGLYARG
31   THRPHEPROHISGLYILESERLYSGLUTHR
32   ALATYRSERGLUSERVALLYSLEULEUGLN
33   LYSILELEUGLUGLUASPGLUARGLYSILE
34   ARGARGILEGLYVALARGPHESERLYSPHE
35   ILEGLUALAILEGLYLEUASPLYSPHEPHE
36   ASPTHRLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Y-family DNA polymerase IV, Dpo4, [U-13C; U-15N; U-2H], 0.6 mM; TRIS, [U-2H], 50 mM; glycerol, [U-2H], 1 mM; D2O, [U-2H], 8%

sample_conditions_1: ionic strength: 0.075 M; pH: 7.5; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks